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Preparation method of isomalto-oligosaccharide

A technology of isomaltooligosaccharide and stirrer, which is applied in the field of preparation of isomaltooligosaccharide, can solve the problems of high content and low content of oligosaccharide, and achieve the effect of taking into account the enzyme activity

Pending Publication Date: 2020-11-17
安徽民祯生物工程有限公司
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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a preparation method of isomaltooligosaccharide, which solves the problem that the content of glucose, maltose and isomaltose are too high in the existing production of isomaltooligosaccharide, while panose, isomaltose Low content of oligosaccharides above maltotriose and tetrasaccharide

Method used

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  • Preparation method of isomalto-oligosaccharide

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] see figure 1 , the embodiment of the present invention provides a technical solution: a preparation method of isomaltooligosaccharide, the preparation method specifically includes the following steps:

[0027] Step 1. Use distilled water and cornstarch to prepare starch milk with a concentration of 30-40% in the mixer, add calcium chloride to the starch milk to adjust the pH value to 6.2-6.4, add high-temperature α-amylase and pass it through an ultraso...

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Abstract

The invention discloses a preparation method of isomalto-oligosacharide. The preparation method specifically comprises the following steps: 1, preparing starch milk with the concentration of 30-40% byusing distilled water and corn starch in a stirrer, adding calcium chloride into the starch milk to adjust the pH value to 6.2-6.4, adding high-temperature alpha amylase, and sufficiently stirring byusing an ultrasonic stirrer; and step 2, performing heating while stirring, stopping heating when the temperature is heated to 100-110 DEG C, then keeping the temperature for 10-20 minutes, and thencarrying out spray liquefaction on the prepared starch slurry. The invention relates to the technical field of isomalto-oligosaccharide. According to the preparation method of the isomalto-oligosaccharide, the content of IMO (isomalto-oligosaccharide) in the resin is set to be 95.8% instead of 80.7% in the prior art during chromatographic separation, so that the recovery rate of IMO is increased,the separation capacity of the resin is improved, and the content of oligosaccharides such as panose, isomaltotriose and tetraose is increased; and the functionality of the isomalto-oligosaccharide isimproved.

Description

technical field [0001] The invention relates to the technical field of isomaltooligosaccharides, in particular to a preparation method of isomaltooligosaccharides. Background technique [0002] In nature, isomaltooligosaccharide rarely exists in a free state, but as a component of amylopectin or polysaccharides, it exists in a small amount in some fermented foods such as soy sauce, rice wine or enzymatic glucose syrup. Industrial production of isomaltooligosaccharides from starch requires an enzyme, which is α-glucosidase, also known as glucosyltransferase, or α-glucosidase for short. It can cut α-1,6 glycosidic bonds in the molecular structure of maltose and maltooligosaccharides, and can transfer a free glucose residue to another glucose molecule or α-1 in molecules such as maltose or maltotriose , at position 6, forming isomaltose, isomaltotriose, isomaltotetraose, isomaltopentaose and panose, etc. Maltose is a disaccharide formed by two glucose units linked by α-1,4 gly...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04C12P19/14C12P19/18
CPCC12P19/00C12P19/04C12P19/14C12P19/18
Inventor 宋建民王德海宛荣生黄祥君张琴王颂
Owner 安徽民祯生物工程有限公司
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