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Moisturizing hyaluronic acid combined water-level fermentation whitening liquid extraction process

An extraction process, hyaluronic acid technology, applied in fermentation, biochemical equipment and methods, cosmetics, etc., can solve the problems of incomplete removal of protein and bacteria, complicated extraction process, and decreased dissolved oxygen, so as to reduce bacteria and protein content, increase the whitening effect, enhance the fragrance effect

Inactive Publication Date: 2020-11-17
广州海纳百川品牌管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hyaluronic acid has a unique protective effect on the skin, which can keep the skin moisturized, smooth, delicate, soft, and elastic. The preparation and extraction process is relatively complicated. During the extraction process, sugars and group A streptococci are required to be fermented, but usually the sugars in the fermentation medium during the fermentation process are added at one time. With the generation of hyaluronic acid , the viscosity of the fermentation liquid increases, resulting in a decrease in dissolved oxygen and a decrease in yield. At the same time, the fermentation raw liquid contains bacteria and proteins, which need to be filtered to remove bacteria and proteins. A single filtration will cause incomplete removal of proteins and bacteria. At the same time, after the hyaluronic acid is prepared by fermentation, it has no taste, and it only has anti-wrinkle and skin-improving effects, and is poor in whitening

Method used

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  • Moisturizing hyaluronic acid combined water-level fermentation whitening liquid extraction process

Examples

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Effect test

Embodiment 1

[0032] A process for extracting hydrating hyaluronic acid combined water level fermentation whitening liquid, comprising the following steps: Step 1, raw material preparation; Step 2, bacterial strain cultivation; Step 3, seed cultivation; Step 4, shake flask cultivation; Step 5, fermentation cultivation; Step 6, Ethanol precipitation; Step 7, add water to dissolve; Step 8, solution filtration; Step 9, secondary filtration; Step 10, add additives; Step 11, finished product storage;

[0033] Wherein in above-mentioned step one, at first solid culture medium, seed culture medium, fermentation medium and group A streptococcus are prepared, and seed culture medium is respectively 10 parts by weight of peptone, 10 parts of beef extract, 1 Parts of sodium chloride, 2 parts of dipotassium hydrogen phosphate and 0.3 parts of magnesium sulfate are mixed, and the fermentation medium is respectively 10 parts by weight of sucrose, 3 parts of glucose, 8 parts of peptone, 5 parts of Yeast p...

Embodiment 2

[0045] A process for extracting hydrating hyaluronic acid combined water level fermentation whitening liquid, comprising the following steps: Step 1, raw material preparation; Step 2, bacterial strain cultivation; Step 3, seed cultivation; Step 4, shake flask cultivation; Step 5, fermentation cultivation; Step 6, Ethanol precipitation; Step 7, add water to dissolve; Step 8, solution filtration; Step 9, secondary filtration; Step 10, add additives; Step 11, finished product storage;

[0046] Wherein in above-mentioned step one, at first solid culture medium, seed culture medium, fermentation medium and group A streptococcus are prepared, and seed culture medium is respectively the peptone of 15 parts, the beef extract of 20 parts, the beef extract of 3 parts by weight respectively. Parts of sodium chloride, 3 parts of dipotassium hydrogen phosphate and 0.5 parts of magnesium sulfate are mixed, and the fermentation medium is respectively 20 parts by weight of sucrose, 10 parts of...

Embodiment 3

[0058] A process for extracting hydrating hyaluronic acid combined water level fermentation whitening liquid, comprising the following steps: Step 1, raw material preparation; Step 2, bacterial strain cultivation; Step 3, seed cultivation; Step 4, shake flask cultivation; Step 5, fermentation cultivation; Step 6, Ethanol precipitation; Step 7, add water to dissolve; Step 8, solution filtration; Step 9, secondary filtration; Step 10, add additives; Step 11, finished product storage;

[0059] Wherein in above-mentioned step one, at first solid culture medium, seed culture medium, fermentation medium and group A streptococcus are prepared, and seed culture medium is respectively the peptone of 12.5 parts, the beef extract of 15 parts, the beef extract of 2 parts by weight respectively. Parts of sodium chloride, 2.5 parts of dipotassium hydrogen phosphate and 0.4 parts of magnesium sulfate are mixed, and the fermentation medium is respectively 15 parts by weight of sucrose, 6 parts...

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Abstract

The invention discloses a moisturizing hyaluronic acid combined water-level fermentation whitening liquid extraction process. The moisturizing hyaluronic acid combined water-level fermentation whitening liquid comprises the following steps of 1, raw material preparartion; 2, strain culture; 3, seed culture; 4, shake-flask culture; 5, fermentation culture; 6, ethanol precipitation; 7, adding waterfor dissolving; 8, filtering the solution; 9, carrying out secondary filtration; 10, adding an auxiliary agent; and 11, finished product storage. According to the moisturizing hyaluronic acid combinedwater-level fermentation whitening liquid, glucose and cane sugar are supplemented in batches in the fermentation process, so that the phenomenon of reduction of the product conversion rate caused bymassive growth of cells and excessive oxygen consumption due to excessive feeding at a time is avoided, the yield and conversion rate of hyaluronic acid are favorably improved, the contents of thalliand proteins in the solution are greatly reduced through two times of filtration, the quality of a product is improved, and lemon essences and the aloe juice are added, so that the overall fragranceof the solution is improved, meanwhile, the whitening effect of the solution is improved, and the beautifying effect of the product is improved.

Description

technical field [0001] The invention relates to the technical field of extraction technology, in particular to an extraction technology of water replenishing hyaluronic acid combined water level fermentation whitening liquid. Background technique [0002] Hyaluronic acid is a disaccharide unit composed of D-glucuronic acid and N-acetylglucosamine, also known as uronic acid and hyaluronic acid. The basic structure is composed of two disaccharide units D-glucuronic acid and N-acetylglucosamine. A large polysaccharide composed of glycosamines, unlike other mucopolysaccharides, it does not contain sulfur. [0003] Hyaluronic acid has a unique protective effect on the skin, which can keep the skin moisturized, smooth, delicate, soft, and elastic. The preparation and extraction process is relatively complicated. During the extraction process, sugars and group A streptococci are required to be fermented, but usually the sugars in the fermentation medium during the fermentation pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/9794A61K8/9789A61Q19/02C12P19/26C12R1/46
CPCA61K8/735A61K8/9794A61K8/9789A61Q19/02C12P19/26A61K2800/85
Inventor 傅海华徐奔
Owner 广州海纳百川品牌管理有限公司
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