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Whitening composition containing apple stem cells and application thereof

An apple stem cell and composition technology, applied in the field of biological preparations, can solve uncommon problems and achieve the effect of high safety

Inactive Publication Date: 2020-11-13
上海熙承生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Apple stem cells are a kind of plant stem cells, but the research on applying apple stem cells to whitening skin care products is not common at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The whitening composition containing apple stem cells is composed of the following ingredients in parts by weight: 40 parts of apple stem cells; 25 parts of aloe extract; 15 parts of Yuyejinhua extract.

[0026] The described apple stem cells are prepared by the following method: (1) take chopped apple peels, inoculate them in MS medium after disinfection, first culture them under light, and then culture them in the dark until the concentration of stem cells is 4×10 6 cells / mL; the culture conditions are as follows: the control temperature is 18°C; the control light intensity is 2500Lux, and the light time is 8h; the dark culture time is 12h; Dry to get the apple stem cells.

[0027] The aloe extract is prepared by the following method: (1) take aloe and add 95% ethanol for heating and reflux extraction, concentrate and dry the extract to obtain the aloe ethanol extract; the weight dosage of the ethanol is 15 times the weight of the aloe (2) Suspend the alcoholic extra...

Embodiment 2

[0030] The whitening composition containing apple stem cells consists of the following ingredients in parts by weight: 30 parts of apple stem cells; 30 parts of aloe extract; 10 parts of Jade leaf golden flower extract.

[0031] The described apple stem cells are prepared by the following method: (1) Take chopped apple peels, inoculate them in MS medium after disinfection, first culture them under light, and then culture them in the dark until the concentration of stem cells is 5×10 6 cells / mL; the culture conditions are as follows: the control temperature is 20°C; the control light intensity is 2000Lux, and the light time is 10h; the dark culture time is 10h; Dry to get the apple stem cells.

[0032] The aloe extract is prepared by the following method: (1) take aloe and add 95% ethanol for heating and reflux extraction, concentrate and dry the extract to obtain the aloe alcohol extract; the weight dosage of the ethanol is 10 times the weight of the aloe (2) Suspend the alco...

Embodiment 3

[0035] The whitening composition containing apple stem cells is composed of the following ingredients in parts by weight: 50 parts of apple stem cells; 20 parts of aloe extract; 10 parts of Yuyejinhua extract.

[0036] The described apple stem cells are prepared by the following method: (1) Take chopped apple peels, inoculate them in MS medium after disinfection, first culture them under light, and then culture them in the dark until the concentration of stem cells is 3×10 6 cells / mL; the culture conditions are as follows: the control temperature is 16°C; the control light intensity is 1500Lux, and the light time is 8h; the dark culture time is 12h; Dry to get the apple stem cells.

[0037] The aloe extract is prepared by the following method: (1) take aloe and add 95% ethanol for heating and reflux extraction, and concentrate and dry the extract to obtain the aloe alcohol extract; the weight consumption of the ethanol is 12 times the weight of the aloe (2) Suspend the alcoho...

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Abstract

The invention belongs to the technical field of biological agents, and particularly discloses a whitening composition containing apple stem cells and application thereof. The whitening composition containing the apple stem cells is prepared from 30 to 50 parts of the apple stem cells; 20 to 30 parts of an aloe extract; and 10 to 20 parts of mussaenda pubescens extract. The apple stem cells are prepared by the following method: (1) taking shredded apple peel, disinfecting, inoculating into an MS culture medium, carrying out illumination culture, and then carrying out dark culture until the stemcell concentration is (3-5) * 10 < 6 > / mL, wherein the culture conditions are as follows: the temperature is controlled to be 15-20 DEG C, the illumination intensity is controlled to range from 1500Lux to 3000 Lux, and the illumination time ranges from 5 h to 10 h, and the dark culture time is 10-15 hours; and (2) separating the stem cells cultured in the step (1), and freeze-drying to obtain the apple stem cells. The composition has a remarkable whitening effect, and meanwhile, the whitening composition is a natural source and has relatively high safety when being applied to skin care products.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a whitening composition containing apple stem cells and an application thereof. Background technique [0002] Plant stem cells have the ability of self-renewal and division. It is the core root of plant vitality and is called "possessing eternal vitality". With the development of plant stem cell technology, it is one of the research directions of plant stem cells to isolate and cultivate plant stem cells from various plants and apply them in the fields of medicine and skin care products. [0003] Apple stem cells are a kind of plant stem cells, but the current research on applying apple stem cells to whitening skin care products is not common. At present, most of the existing whitening skin care products are chemical products. As women begin to pay attention to the quality and safety of skin care products, whitening skin care products of biological or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9794A61K8/9789A61Q19/02
CPCA61K8/99A61K2800/592A61K2800/5922A61K2800/84A61Q19/02A61K8/9789A61K8/9794
Inventor 金京姬
Owner 上海熙承生物科技有限公司
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