Early screening and diagnosis method for colorectal cancer based on miRNAs of rectal mucosal epithelial cell brushing

A technology based on epithelial cells and rectal mucosa, applied in the fields of biomedical research and clinical detection and diagnosis, can solve the problems of easy degradation of mRNA, difficulty in preservation, etc., and achieve the effect of low detection price, easy promotion, and simple sample processing process

Pending Publication Date: 2020-11-10
承德医学院附属医院
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Problems solved by technology

It is a pity that mRNA is easy to degrade and difficult to preserve, so this method cannot be widely used in clinical practice.

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  • Early screening and diagnosis method for colorectal cancer based on miRNAs of rectal mucosal epithelial cell brushing

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Embodiment Construction

[0026] Diagnosis principle:

[0027] For the first time, it was proved that the abnormal expression of the same miRNA exists in both colorectal cancer tissue and rectal mucosal epithelial cells. The literature library screened the abnormally expressed miRNAs in colorectal cancer, and retrieved 9 colon cancer miRNA expression profiling studies through the PubMed database. Subsequently, the "Venn diagram" software was used to perform cross-analysis on the miRNA expression profile data, and finally four miRNAs abnormally expressed in colorectal cancer tissues were screened out from 9 independent miRNA expression profile results as colorectal cancer screening markers, and were used as markers for colorectal cancer screening. The expression of miRNA-29 / miRNA-96 / miRNA-182 / miRNA-135 in different tissues was verified in five patients with colorectal cancer.

[0028] Preliminary verification of differential expression of miRNA-29 / miRNA-96 / miRNA-182 / miRNA-135 in different parts of the s...

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Abstract

The invention relates to an early screening and diagnosis method for colorectal cancer based on miRNAs of rectal mucosal epithelial cell brushing. The method comprises the following steps: s1, rectalswab collection; s2, swab collection of tumor sites; s3, total miRNA extraction in the cell, and reverse transcription; s4, detection by using real-time PCR method; and s5, statistical analysis. According to the present invention, the method is non-invasive and has good patient compliance: rectal mucosal epithelial cells are obtained by brushing during anal brushing; the sample processing processis simple, miRNA can be applied to degraded samples and is not easily affected by food and drugs, and the detection results are highly reliable; gene changes can occur in the early stage of colorectalcancer, so the method is suitable for early screening; and the technology is mature, the detection is low in price, and the method is prone to popularization, and is used for large-scale population screening.

Description

technical field [0001] The invention relates to the technical fields of biomedical research and clinical detection and diagnosis, in particular to a miRNAs colorectal cancer early screening and diagnosis method based on rectal mucosal epithelial cell brushing. Background technique [0002] With the improvement of people's living standards in our country, changes in eating habits and structure, coupled with the impact of population aging trend, the incidence and mortality of colorectal cancer are increasing year by year. According to statistics, as many as 400,000 patients died of colorectal cancer in my country in 2015. Since the early symptoms of colorectal cancer are not obvious, most patients often come to the hospital several weeks or even months after the onset of symptoms, and most of them have developed to an advanced stage, missing the best time for treatment. [0003] At present, worldwide, colonoscopy is known as the "gold" standard for the diagnosis of colorectal...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 何培元李炳庆侯志平尹春英王鑫李思锦
Owner 承德医学院附属医院
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