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Application of galactotetraose sulfate and derivatives thereof in preparation of drugs for resisting epithelial cell adhesion

A technology of epithelial cells and lactotetraose, which is applied in the field of medicine and achieves the effects of broad market application prospects, high yield and simple preparation process

Pending Publication Date: 2020-11-10
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that gene mutations that interfere with FAK-tailin junctions inhibit tailin cleavage, thereby inhibiting effective focal adhesion turnover[19]

Method used

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  • Application of galactotetraose sulfate and derivatives thereof in preparation of drugs for resisting epithelial cell adhesion
  • Application of galactotetraose sulfate and derivatives thereof in preparation of drugs for resisting epithelial cell adhesion
  • Application of galactotetraose sulfate and derivatives thereof in preparation of drugs for resisting epithelial cell adhesion

Examples

Experimental program
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Embodiment 1

[0056] Example 1: Extraction, preparation and analysis of galactotetraose sulfate

[0057] Dried Songzao in the sun, ground into powder, degreased and stirred in a water bath at a constant temperature of 50 degrees Celsius for 10 hours; after concentration, 70% and 80% ethanol were graded and precipitated; the precipitate was collected and freeze-dried; the powder was condensed with 0.05-0.1mol / L dilute hydrochloric acid at 58 degrees Celsius Carry out constant temperature degradation, 58 degrees Celsius, 3 hours; Concentrate the solution, ethanol precipitation, take the supernatant and concentrate it with a rotator, and then conduct desalting treatment through a gel chromatography column; then use Bio-Gel P6 chromatography column to separate and purify; collect The peak part was lyophilized to obtain galactotetraose sulfate.

[0058] The obtained galactotetraose sulfate structural formula is as follows:

[0059]

[0060] Take the sample and add 0.05M NaBH4 to react overni...

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Abstract

The invention provides application of galactotetraose sulfate and derivatives thereof in preparation of drugs for resisting epithelial cell adhesion. The galactotetraose sulfate or the derivatives thereof are applied to the drugs for resisting the epithelial cell adhesion, or the galactotetraose sulfate or the derivatives thereof are applied to a functional food for resisting the epithelial cell adhesion. The galactotetraose sulfate can achieve target binding with focal adhesion kinas (FAK). In this way, the adhesion capability of epithelial cells is effectively inhibited.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of galactotetraose sulfate and derivatives thereof in the preparation of anti-epithelial cell adhesion drugs. Background technique [0002] Focal adhesion kinase (FAK) is an intracellular non-receptor tyrosine kinase that was first discovered in transfected V-Src chicken embryo fibroblasts[1]. FAK is highly expressed in most tissues, and its protein sequence has high homology in many species (mouse, toad, human, etc.). FAK is the intersection of multiple signal transduction pathways in cells, and participates in multiple biological processes such as tumor formation, proliferation, metastasis and apoptosis, and cardiovascular diseases. In this paper, the main signal transduction pathways, biological effects and research progress of FAK as an anti-tumor target are reviewed in recent years. The relative molecular mass of FAK is 125kDa, and its structur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P35/00A23L33/125
CPCA61K31/7024A61P35/00A23L33/125A23V2002/00A23V2200/308A23V2250/608
Inventor 杜衍晓王凤娟李侠
Owner QINGDAO CENT HOSPITAL
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