Treatment method for increasing germination rate of tilia miqueliana seeds
A treatment method and technology of germination rate, applied in the field of treatment to improve the germination rate of linden seeds in Nanjing, can solve the problems of germination taking 2-3 years, affecting the planting and promotion of seedlings, hindering the reproduction of linden in Nanjing, etc., so as to facilitate planting and promotion. , the effect of enhancing resistance and improving germination rate
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Embodiment 1
[0026] A treatment method for improving the germination rate of Nanjing Tilia seeds comprises the following steps:
[0027] Step 1: Seed pretreatment, after collecting the seeds, soak them in clean water;
[0028] Step 2: Seed disinfection treatment, take out the soaked seeds in step 1, rinse the seeds with sterile water, soak them in 75% ethanol for 40 seconds, and then put them in 0.3% potassium permanganate Soak and disinfect for 3 minutes, rinse with sterile water several times after taking it out, and use filter paper to absorb the floating water on the surface;
[0029] Step 3: Seed soaking treatment, soaking the sterilized seeds in step 2 in a gibberellin hormone solution of 500mg / L-1000mg / L for 6h;
[0030] Step: 4: Prepare the culture medium. While soaking the seeds in step 3, mix the culture medium, adjust the pH value to 5.8-6.0, heat the culture medium in a microwave oven until it boils, and use a sterilized glass rod to carry out Stir, after the stirring is comp...
Embodiment 2
[0036] A treatment method for improving the germination rate of Nanjing Tilia seeds comprises the following steps:
[0037] Step 1: Seed pretreatment, after collecting the seeds, soak them in clean water;
[0038] Step 2: Seed disinfection treatment, take out the soaked seeds in step 1, rinse the seeds with sterile water, soak them in 75% ethanol for 40 seconds, and then put them in 0.3% potassium permanganate Soak and disinfect for 3 minutes, rinse with sterile water several times after taking it out, and use filter paper to absorb the floating water on the surface;
[0039] Step 3: Seed soaking treatment, soaking the sterilized seeds in step 2 in a gibberellin hormone solution of 500mg / L-1000mg / L for 6h;
[0040] Step: 4: Prepare the culture medium. While soaking the seeds in step 3, mix the culture medium, adjust the pH value to 5.8-6.0, heat the culture medium in a microwave oven until it boils, and use a sterilized glass rod to carry out Stir, after the stirring is comp...
Embodiment 3
[0047] A treatment method for improving the germination rate of Nanjing Tilia seeds comprises the following steps:
[0048] Step 1: Seed pretreatment, after collecting the seeds, soak them in clean water;
[0049] Step 2: Seed disinfection treatment, take out the soaked seeds in step 1, rinse the seeds with sterile water, soak them in 75% ethanol for 40 seconds, and then put them in 0.3% potassium permanganate Soak and disinfect for 3 minutes, rinse with sterile water several times after taking it out, and use filter paper to absorb the floating water on the surface;
[0050] Step 3: Seed soaking treatment, soaking the sterilized seeds in step 2 in a gibberellin hormone solution of 500mg / L-1000mg / L for 6h;
[0051] Step: 4: Prepare the culture medium. While soaking the seeds in step 3, mix the culture medium, adjust the pH value to 5.8-6.0, heat the culture medium in a microwave oven until it boils, and use a sterilized glass rod to carry out Stir, after the stirring is comp...
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