Method for preparing genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate and application in preparation of antitumor drugs

A ferric ammonium citrate, urinary system technology, applied in the field of preparation of anti-tumor drugs, can solve the problems of low model stability and high toxicity, and achieve the effect of promoting ferroptosis and increasing expression

Active Publication Date: 2020-11-06
刘特
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Problems solved by technology

The development of drugs that cause ferroptosis in tumor cells of the genitourinary system is very meaningful for the realization of its clinical treatment, but the current Fe-containing 2+ Drugs are highly toxic, and the amount of these drugs varies greatly on different cells, so the stability of the model constructed with these drugs is not high

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  • Method for preparing genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate and application in preparation of antitumor drugs
  • Method for preparing genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate and application in preparation of antitumor drugs
  • Method for preparing genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate and application in preparation of antitumor drugs

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[0020] The invention provides a method for preparing a ferroptosis model of genitourinary system tumor cells based on ferric ammonium citrate, comprising the following steps: when the density of cultured tumor cells reaches 65-75%, adding ferric ammonium citrate and continuing to cultivate for 20-30 hours , to get the ferroptosis model. Ammonium ferric citrate, also known as ammonium ferric citrate (AFC), 2-hydroxy-1,2,3-propane tricarboxylic acid ammonium iron, is a double salt of ferric citrate and ammonium citrate ( figure 1 ). AFC is usually added to dairy products, wheat flour for bread, biscuits, milk powder, etc., and is used in nutritional supplements (iron fortifiers), and is a very safe chemical substance. That is, AFC is a generally recognized safe food additive grade iron supplement (for the treatment of iron deficiency anemia), which is edible, has a high safety factor, and has little toxic and side effects, while many existing chemotherapeutic drugs are very tox...

Embodiment 1

[0033] 1. Cell culture:

[0034] Human genitourinary system tumor cell lines including breast cancer cell MCF7, ovarian cancer cell SK-OV-3, prostate cancer cell 22Rv1, and kidney cancer cell A-498 were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. The above cells were cultured in McCOY's 5A (SIGMA, M4892) medium containing NaHCO 3 2.2g / L, high-quality fetal bovine serum 10%. The culture environment is gas phase: air, 95%; carbon dioxide, 5%. Temperature: 37°C.

[0035] 2.MTT experiment:

[0036] Ferric ammonium citrate was purchased from SIGMA. It was made up to a storage concentration of 100 mg / ml in sterile PBS and sterilized by suction through a filter with a pore size of 0.22 μm. Take 2×10 3 / ml Tumor cells in good growth state were seeded in 96-well cell culture plates. On the next day, AFC was added so that the concentrations were 0.001, 0.01, 0.1, and 1.0 mg / ml respectively. Three parallel c...

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Abstract

The invention relates to a method for preparing a genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate and application in preparation of antitumor drugs, and belongs to the technical field of antitumor drugs. The invention provides the method for preparing the genitourinary system tumor cell ferroptosis model based on ferric ammonium citrate. The method comprises thefollowing steps: when the density of cultured tumor cells reaches 65-75%, adding ferric ammonium citrate, and continuing to culture for 20-30 hours to obtain the ferroptosis model. The way of treatinggenitourinary system tumor cells by using the ferric ammonium citrate is a very simple, convenient and efficient method for preparing the a ferroptosis tumor cell model, and the anti-tumor activity of the ferric ammonium citrate provides a theoretical basis for subsequent research and development and clinical treatment.

Description

technical field [0001] The invention relates to the technical field of antitumor drugs, in particular to a method for preparing a ferroptosis model of genitourinary system tumor cells based on ferric ammonium citrate and an application for preparing antitumor drugs. Background technique [0002] Genitourinary system tumors seriously endanger human life and health, and the existing radiotherapy, chemotherapy and surgical treatment are not ideal. In recent years, the incidence of genitourinary system tumors has shown a rapid increase year by year, especially breast cancer, ovarian cancer, prostate cancer and kidney cancer. Usually, most genitourinary system tumors do not have many physical symptoms in the early stage. Once discovered, they have entered the middle and late stages. At present, many chemotherapeutic drugs have serious side effects. While killing tumor cells, they also cause great damage to normal cells in the body. Therefore, it is urgent to develop some broad-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A61K33/26A61P35/00
CPCC12N5/0693A61K33/26A61P35/00C12N2500/30
Inventor 刘特黄永毅龚张斌白浩然沈洪亮
Owner 刘特
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