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Pyrethroid insecticide degrading strain as well as microbial inoculum and degrading process thereof

A technology for pyrethroids and insecticides, which is applied in the field of pyrethroid insecticides degradation strains and their bacterial agents and degradation technology, can solve the problems of different efficiency and metabolic activity of insecticides, and achieve great application value Effect

Active Publication Date: 2020-11-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing reported allethrin-degrading microorganisms include Bacillus megalosporus strain HLJ7 (CN111004736A), Sphingomonas trueperi strain CW3, Staphylococcus succinus strain HLJ-10, non-decarboxylation Leclercia adecarboxylata strain Y4 and Alcaligenes sp. strain A-6; however, due to the different genetic characteristics and metabolic activities of the degrading microorganisms themselves, the degradation of pyrethroid insecticides Efficiency is also different

Method used

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  • Pyrethroid insecticide degrading strain as well as microbial inoculum and degrading process thereof
  • Pyrethroid insecticide degrading strain as well as microbial inoculum and degrading process thereof
  • Pyrethroid insecticide degrading strain as well as microbial inoculum and degrading process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation and Identification of Pseudomonas nitroreducens CW-7

[0045] 1. Screening and isolation of allethrin-degrading strains

[0046] Collect activated sludge from the wastewater treatment tank of a pesticide factory in Nanning, Guangxi, weigh 5g of activated sludge samples and add to 50mL of the above-mentioned MSM liquid medium containing allethrin (50mg / L). After culturing at 30°C and 200r / min for 7 days, the mass concentration of pesticides was increased from 50mg / L to 100mg / L, 200mg / L, 400mg / L, and 800mg / L in sequence at 10% inoculum size each time for continuous enrichment culture . Then, the culture fluid transferred for 4 times was serially diluted and spread on MSM solid plates containing 400 and 800 mg / L allethrin, and cultured upside down at 30°C for 2 days. After a single colony grows on the plate, a single colony is picked and streaked on LB solid medium for purification, and then its degradation effect is verified by high performance liqui...

Embodiment 2

[0056] The preparation of embodiment 2 nitroreductor pseudomonas CW-7 degrading bacteria agent

[0057] The production process of using the above-mentioned Pseudomonas nitroreductors CW-7 to prepare the degrading bacterial agent is: slant strain-shake flask seed liquid-seed tank cultivation-production tank fermentation-degrading bacterial agent (dosage form can be suspension or powder) ). The specific method is as follows:

[0058] (1) Activate the nitroreductor Pseudomonas CW-7 strain on the LB solid plate, and inoculate it on the inclined surface of the LB test tube for later use.

[0059] (2) Inoculate the test tube seed of Pseudomonas nitroreductans CW-7 into a 1000mL shake flask containing 250mL LB medium, shake at a constant temperature of 30°C to the logarithmic phase, and inoculate the obtained bacterial liquid into the seed tank, and the seed tank The medium is filled with sterilized seed culture medium, and the filling volume is 70%. Inoculate the cultivated shake...

Embodiment 3

[0061] The degradation process condition of embodiment 3 nitroreductor pseudomonas CW-7 degrading bacterial agent

[0062] 1. Experimental method

[0063] Nitro-reducing Pseudomonas CW-7 was subjected to a single-factor degradation test, and the nitro To determine the degradation rate of Pseudomonas reductans CW-7 degrading bacterial agent, to determine the key factors affecting the degradation rate of nitroreductant Pseudomonas CW-7 degrading bacterial agent.

[0064] SAS 9.0 statistical software was used to carry out experimental design according to the Box-Behnken design principle of response surface method (Table 1), and the key factors were pH value (A), temperature (B) and initial concentration of pyrethroid insecticides (C) variable, the response value is the degradation rate of allethrin (Y 1 ), establish a multivariate quadratic regression equation, carry out drawing analysis according to the multivariate quadratic regression equation, and obtain the response surfac...

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Abstract

The invention discloses a pyrethroid insecticide degrading strain as well as a microbial inoculum and a degrading process thereof. The invention obtains pseudomonas nitroreducens CW-7 through screening and separation, the strain is preserved in Guangdong province microbial strain preservation center on June 17, 2020, and the preservation number is GDMCC No. 61063. The strain has broad spectrum fordegrading pyrethroid insecticides and can be used for repairing natural environments such as water bodies and soil polluted by the pyrethroid insecticides, and the residual quantity of the pyrethroidinsecticides in the water bodies and the soil can be reduced by more than 95% after directly applying the strain for 7 days; and the prepared excellent degrading bacterium agent is applied to bioremediation of environments such as water bodies and soil polluted by the pyrethroid insecticides, provides a new microbial resource for bioremediation of pesticide environmental pollution, enriches a germplasm resource library of pesticide degrading bacteria, and provides a theoretical basis and a practical basis for developing a new technology for treating pyrethroid insecticide pollution such as allethrin.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to a pyrethroid insecticide-degrading bacterial strain, bacterial agent and degradation process thereof. Background technique [0002] Pyrethroid insecticides are a class of pesticides synthesized by imitating the chemical structure of natural pyrethrins. They have the characteristics of wide insecticidal spectrum, high efficiency, low toxicity and low residue. They have been widely used in agriculture and are often used in Controls leaf-eating and fruit-eating pests of fruit trees and vegetables. In addition, it has also been widely used in household insecticides, such as the control of mosquitoes, livestock parasites and cockroaches. Its usage ranks second in the international pesticide market and continues to grow . Among them, allethrin is the first commercial pyrethroid pesticide synthesized by American Milton S. Schechter in 1949, and is widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/38C02F101/36C02F101/34
CPCC02F3/34B09C1/10C02F2101/306C02F2101/38C02F2101/36C02F2101/34C12R2001/38C12N1/205Y02A50/30
Inventor 陈少华张文平潘卡·巴特黄耀华张育茗林子秋庞诗梅
Owner SOUTH CHINA AGRI UNIV
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