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Excrement sampling device for DNA methylation detection, excrement preserving fluid and excrement sampling method

The technology of a sampling device and a preservation solution, which is applied in the field of DNA methylation detection stool sampling devices, can solve the problems of small amount of collection and different amounts of excrement, and achieve the effects of improving preservation, reliability and effectiveness.

Pending Publication Date: 2020-11-03
钟玙沄
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common feces collection devices on the market are mainly used for fecal occult blood tests. The main problems are: 1) The amount of collection is small; 2) Due to the viscosity of feces, the amount taken each time is different.

Method used

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  • Excrement sampling device for DNA methylation detection, excrement preserving fluid and excrement sampling method
  • Excrement sampling device for DNA methylation detection, excrement preserving fluid and excrement sampling method
  • Excrement sampling device for DNA methylation detection, excrement preserving fluid and excrement sampling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 DNA methylation detection preparation of feces preservation solution

[0050] A DNA methylation detection feces preservation solution, the concentration of the components of the feces preservation solution is: Tris hydrochloric acid buffer solution 10-1000mM, sodium chloride 1-10mM, ammonium sulfate 1-100mM, ethylene glycol Aminetetraacetic acid 1-100mM.

[0051] The preparation method of the DNA methylation detection feces preservation solution, the steps are as follows:

[0052] Dissolve tris hydrochloride buffer, sodium chloride, ammonium sulfate, and ethylenediaminetetraacetic acid in pure water and dilute to the above concentrations;

[0053] Mix to obtain the DNA methylation detection stool preservation solution.

Embodiment 2

[0054] Example 2 DNA methylation detection preparation of feces preservation solution

[0055] A DNA methylation detection feces preservation solution, the concentration of the components of the feces preservation solution is: Tris hydrochloric acid buffer solution 50-150mM, sodium chloride 1-10mM, ammonium sulfate 1-100mM, ethylene glycol Aminetetraacetic acid 1-100mM.

[0056] The preparation method of the DNA methylation detection feces preservation solution is shown in Example 1.

Embodiment 3

[0057] Example 3 A DNA methylation detection stool sampling device

[0058] Such as figure 1 As shown, the DNA methylation detection stool sampling device includes:

[0059] Two-way through the cylinder;

[0060] There is an ejector rod penetrating through the barrel;

[0061] One end of the ejector rod located in the cylinder is fixedly connected with a piston;

[0062] There is a slot on the ejector rod and the inner wall of the cylinder to limit the range of movement of the piston;

[0063] The piston is in sliding connection with the inner wall of the barrel.

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Abstract

The invention discloses a excrement sampling device for DNA methylation detection, excrement preserving fluid and an excrement sampling method. The excrement sampling device for DNA methylation detection provided by the invention comprises a bidirectional-penetrated cylinder, an ejector rod is arranged in the cylinder in a penetrating mode, a piston is fixedly connected to one end in the cylinder,of the ejector rod, and the piston is in sliding connection with the inner side wall of the cylinder. An excrement collecting device and the preserving fluid provided by the invention fill up the blank of products in the field in the current market, can effectively preserve DNA and methylation characteristics in the DNA, can also improve the excrement sampling reliability and effectiveness for DNA methylation detection, and further help for large-scale intestinal cancer excrement DNA methylation screening is provided.

Description

technical field [0001] The invention relates to the field of stool molecular detection, in particular to a DNA methylation detection stool sampling device, a preservation solution and a method thereof. Background technique [0002] Colorectal cancer is the fifth most common malignancy in terms of morbidity and mortality in my country. Early diagnosis and screening of colorectal cancer can effectively reduce the mortality of malignant tumors. Fecal DNA methylation detection is a non-invasive, highly sensitive, and highly specific detection method, which is significantly better than the current colonoscopy and fecal occult blood test. Its effectiveness has been written into the US colorectal cancer screening guidelines and Chinese colorectal cancer screening experts In consensus. [0003] The accuracy of stool DNA methylation detection is affected to a certain extent by the collection of stool samples and the preservation of DNA methylation features in them. At present, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/24C12Q1/6886C12M1/26
CPCC12Q1/24C12Q1/6886C12Q2600/154
Inventor 钟玙沄
Owner 钟玙沄
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