Preparation method and system for producing important precursor ATC of L-cysteine by enzymic method

A technology of cysteine ​​and preparation equipment, which is applied in the field of biomedicine, can solve the problem of only 70%-80%, and achieve the effects of low environmental pollution, high comprehensive yield, and mild reaction conditions

Pending Publication Date: 2020-11-03
湖北远大生物技术有限公司
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  • Description
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  • Application Information

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Problems solved by technology

In general ATC synthesis, the industrialized large-scale yield is usually only 70%-80%

Method used

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  • Preparation method and system for producing important precursor ATC of L-cysteine by enzymic method
  • Preparation method and system for producing important precursor ATC of L-cysteine by enzymic method
  • Preparation method and system for producing important precursor ATC of L-cysteine by enzymic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] In this example, ATC was prepared according to the following method, the system see Figure 4 .

[0084] 1. Turn on the refrigeration device to cool down, add 200kg of methyl dichloropropionate into the 2T reaction tank, add 300kg of tap water, the liquid volume is about 500L, cool down to 0°C, slowly add 32% sodium hydroxide solution dropwise, the reaction temperature The temperature is controlled below 10°C, and the dropwise addition time is about 1.5-2h. After the dropwise addition is completed, stir slowly for 30 minutes. Then add about 100kg of thiourea, continue to control the reaction temperature below 10°C, slowly add concentrated hydrochloric acid dropwise, continue to keep the temperature below 10°C, dropwise for 30-50min, after the addition is complete, heat up to 30-35°C and keep warm Reaction 4.5-5h. After the heat preservation is completed, continue to slowly add the sodium carbonate solution dropwise for 1.5-2 hours. After the dropwise addition is compl...

Embodiment 2

[0089] In this example, ATC was prepared according to the following method.

[0090] 1. Turn on the refrigeration device to cool down, add 200kg of methyl dichloropropionate into the 2T reaction tank, add 300kg of tap water, the liquid volume is about 500L, cool down to 0°C, slowly add 32% sodium hydroxide solution dropwise, the reaction temperature The temperature is controlled below 10°C, and the dropwise addition time is about 1.5-2h. After the dropwise addition is completed, stir slowly for 30 minutes. Then add about 100kg of thiourea, continue to control the reaction temperature below 10°C, slowly add concentrated hydrochloric acid dropwise, continue to keep the temperature below 10°C, dropwise for 30-50min, after the addition is complete, heat up to 30-35°C and keep warm Reaction 4.5-5h. After the heat preservation is completed, continue to slowly add the sodium carbonate solution dropwise for 1.5-2 hours. After the dropwise addition is completed, raise the temperature ...

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Abstract

The invention provides a preparation method of an important precursor ATC for producing Lcysteine by an enzymic method, which comprises the following steps: (1) preparing ATC to obtain ATC-containingmother liquor; (2) carrying out first evaporation treatment on the mother solution to obtain a first concentrated solution and distilled water; (3) carrying out centrifugal filtration and desalinationon the first concentrated solution, and collecting filtrate; (4) carrying out second evaporation treatment on the filtrate to obtain a second concentrated solution and distilled water; and (5) carrying out crystallization treatment on the second concentrated solution, carrying out centrifugal filtration, and collecting crystals to obtain an ATC purified product, the concentration multiple of thefirst evaporation treatment being 5-6 times. The ATC product prepared by the method is high in yield, the comprehensive yield under the industrial scale can reach 85-90%, the operation is simple, convenient and fast, the cost is low, the wastewater discharge is less, and the method is particularly suitable for industrial production.

Description

technical field [0001] The invention relates to the field of biomedicine. Specifically, the present invention relates to a preparation method and system for enzymatically producing ATC, an important precursor of L-cysteine. Background technique [0002] L-cysteine ​​is a sulfur-containing non-essential amino acid, and its sulfhydryl group has many important physiological functions, and is widely used in the fields of medicine, food and cosmetics. The production methods of L-cysteine ​​mainly include natural product extraction, chemical synthesis and enzymatic conversion. The extraction method is to extract from protein hydrolyzate or hair. This method is often limited by the source of raw materials, and the product is easily mixed with other amino acids, which is not easy to purify. The chemical synthesis method not only has many steps, but also the obtained product is usually a racemate of D-form and L-form, which needs to be resolved to obtain L-cysteine. Because the en...

Claims

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Application Information

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IPC IPC(8): C07D277/18C12P13/12
CPCC07D277/18C12P13/12
Inventor 赵东明林添雄欧阳晖
Owner 湖北远大生物技术有限公司
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