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Rice GS3 mutant gene and molecular marker and application thereof

A technology of molecular markers and mutated genes, applied in genetic engineering, plant gene improvement, applications, etc., can solve the problems of difficult to distinguish differences, increase the time cost of breeding, and can not be produced, etc., to achieve the effect of good primer specificity

Active Publication Date: 2020-10-30
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding mainly uses visual observation to screen target traits, which has strong experience dependence, and there is no unified basis for judgment, which reduces the screening efficiency of breeding, especially for grain shape traits. Due to the small size of rice grains, it is difficult to distinguish the difference with the naked eye.
Moreover, the performance of these traits is environment-dependent, and the expected phenotype cannot be produced in some planting environments, and accurate screening must be achieved through shuttle breeding, which increases the time cost of breeding

Method used

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  • Rice GS3 mutant gene and molecular marker and application thereof
  • Rice GS3 mutant gene and molecular marker and application thereof
  • Rice GS3 mutant gene and molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example relates to the acquisition of GS3 mutant genes and the development of molecular markers.

[0044] At present, multiple grain shape-related genes have been cloned in rice, and GS3 is a site with a more significant effect, which was found in both linkage analysis and association analysis. A C-to-A mutation in the second exon of the gene leads to premature termination of protein translation, loss of gene function, and long-grain phenotype, which is an important mutation type in breeding screening. The gene contains four functional domains, which are OSR, TM, TNFR and VWFC functional domains, and the C to A mutation type is located in the OSR functional domain. During the evolution of rice, different types of beneficial mutations will be produced, and these mutations have gradually been screened and retained by farmers in different countries or regions in history. Combining the structural characteristics of the GS3 gene with different functional domains, we bel...

Embodiment 2

[0062] This example relates to the combination and application of molecular markers related to rice grain type.

[0063] 1. Design of Molecular Marker Combinations

[0064] In addition to GS3, GW5 is another important site that affects grain type. For the known C / A functional variation of the second exon of GS3 and the functional variation of the 1212bp deletion of the GW5 gene, we carried out molecular marker design, and The mode of application of these markers in combination with the markers in Example 1 was explored.

[0065] For the functional variation of GS3 (C→A), we downloaded the corresponding variation information and its flanking sequences from the rice genome variety polymorphism database (http: / / ricevarmap.ncpgr.cn / v2 / ) for primer design , to develop dCaps markers. The marker design method is consistent with the dCaps marker method in Example 1. For the 1212bp deletion mutation of GW5 gene, it can be directly distinguished by the difference of PCR amplification...

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Abstract

The invention discloses a rice GS3 mutant gene and a molecular marker and application thereof. The rice GS3 mutant gene is obtained by deleting three CTC bases behind the 5932 base of the rice GS3 gene, and the mutation site is located at the fifth exon. The invention also discloses a molecular marker aiming at the molecular marker, further discloses a molecular marker combination containingthe molecular marker, and provides application. According to the invention, key variation of grain shape regulation of the fifth exon of the rice GS3 gene is discovered and identified, 3bp deletion mutation participates in regulation of grain shape traits of rice, and the mutation can be used as another screening target for grain shape improvement breeding in the future. The invention develops aneffective dCaps marker and molecular marker combination which can be used for distinguishing and selecting rice grain types, identifying varieties, breeding rice and the like.

Description

technical field [0001] The invention belongs to the field of plant molecular biology, and in particular relates to a rice GS3 mutant gene, its molecular marker and application. Background technique [0002] Rice grain shape is mainly determined by grain length, grain width and aspect ratio, which can ultimately affect rice yield through grain weight. At the same time, grain shape also affects quality traits, including appearance quality, rice milling rate and eating quality. Therefore, in the breeding process, it is necessary to screen the grain type with moderate grain size to achieve the coordinated improvement of yield and quality. In addition, people in different regions of my country have different preferences for the shape of rice. For example, South China prefers long-grain rice, while Jiangsu, Zhejiang and Northeast China prefer short-round rice. Breeding rice varieties with different grain lengths can meet the consumption needs of different markets. Traditional bre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张林卞中刘巧泉张昌泉李钱峰邹怡婷谢东
Owner YANGZHOU UNIV
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