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A kind of rice grain shape related protein glw2 and its coding gene and application

A technology of related proteins and coding genes, which is applied in rice grain shape-related protein GLW2 and its coding genes and applications, can solve the problems of few genes related to rice grain shape and yield traits, unclear and imperfect mechanism of grain shape regulation in rice, etc. question

Inactive Publication Date: 2019-02-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, under the active efforts of scientists all over the world, especially Chinese scientists, the cloning of genes related to rice grain shape and yield traits has made great progress. However, due to the complexity of quantitative trait QTL cloning, the cloned rice grain shape There are still very few genes related to yield traits, and the mechanism of grain shape regulation in rice is still unclear and incomplete

Method used

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  • A kind of rice grain shape related protein glw2 and its coding gene and application
  • A kind of rice grain shape related protein glw2 and its coding gene and application
  • A kind of rice grain shape related protein glw2 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Transgenic test of GLW2 gene rice

[0071] 1. Construction of GLW2 gene overexpression expression vector

[0072] In this example, the expression vector PHB (Mao et al., 2005, PNAS102:12270-12275, commercially available) was used as a rice transgene vector. This vector encodes a bacterial origin of replication (ori), kanamycin resistance gene (Kan r ), hygromycin resistance gene (Hyg r ), herbicide resistance gene (Bar r ), 2×35S promoter, NOS gene termination signal sequence, and multiple cloning site (MCS) for connecting target fragments. The GLW2 gene or its fragments can be inserted into the restriction endonuclease site to construct a transgenic plasmid.

[0073] Synthesize the first-strand cDNA using the RNA of the normal wild-type IR24 variety as a template, and use the oligonucleotides at the 5'and 3'ends of the ORF sequence of the GLW2 gene as PCR primers (SEQ ID NO. 3 and SEQ ID NO. 4) , Amplify with high-fidelity enzyme to obtain the full-length cDNA amp...

Embodiment 2

[0088] Example 2 GLW2 gene function analysis

[0089] The transgenic plants overexpressing rice GLW2 were obtained by the method described in Example 1. They were divided into 2 groups, named OE1-2, OE2-3, and the normal control group. The phenotype of rice grains was observed with the transgenic T1 generation plants. The result is Figure 3~7 As shown, where Figure 4~7 In, ** means significant at the level of 0.01, NS means unintentional control).

[0090] It can be seen from the figure that the grain length, grain width and thousand-grain weight of the transgenic plants with the overexpression gene of GLW2 increased significantly compared with the control. It shows that overexpression of GLW2 will increase the length and width of grains, which in turn leads to an increase in thousand-grain weight.

[0091] The transgenic plants of rice GLW2 knockout were obtained by the method described in Example 1. They were divided into 4 groups, named KO1-1, KO1-2, KO1-5 and KO2-3 respectivel...

Embodiment 3

[0096] Example 3 Phenotype of GLW2 gene mutation (GLW2 natural mutation material)

[0097] The rice variety 307R (preserved in the Resource Center of Rice Research Institute of Sichuan Agricultural University) after the mutation of the GLW2 gene shows that the 307R grains are better than the existing normal rice varieties 527R, IR24 and M63. The grain length, grain width and thousand-grain weight are significantly increased compared with other rice materials ( Picture 12 And Table 4).

[0098] Table 4 Tested value statistics of normal rice varieties and 307R mature grains

[0099]

[0100] Compared with the shortcomings and deficiencies of the prior art, the present invention has the following beneficial effects: the rice grain type control gene (GLW2) of the present invention provides a new technical approach for the grain type regulation of rice and other plants; the GLW2 gene has the effect of removing transgenic components It has a wide range of application prospects; this gene...

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Abstract

The invention provides rice grain shape related protein GLW2 and an encoding gene and application thereof. The protein GLW2 has an amino acid sequence shown as in SEQ ID No. 2 or an equivalently functional amino acid sequence formed by subjecting an amino acid sequence shown in SEQ ID No. 2 to substitution, deletion or addition via one or more amino acid residues; the encoding gene has a nucleotide sequence shown as in SEQ ID No. 1 or a nucleotide sequence that hybridizes with the nucleotide sequence shown in SEQ ID No. 1 under strict conditions. After the encoding gene GLW2 is recombined to an expression vector and converted to rice, overexpression of GLW2 can increase rice grain length and width and increase rice yield.

Description

Technical field [0001] The invention belongs to plant genetic engineering and plant breeding, and particularly relates to a rice grain type-related protein GLW2 and its coding gene and application. Background technique [0002] As an important food crop, rice plays an important role in my country's food security. Continuously increasing rice yield per unit area is a major issue related to the national economy and people's livelihood. Grain type has a huge impact on endosperm structure, rice appearance, thousand-grain weight, seed setting rate, grain number per ear and other traits. It is not only an important indicator of rice quality, but also a key factor in yield increase. With the continuous development of gene mapping populations and the further development of new molecular markers, more rice key trait genes have been mapped and cloned. Research on these new genes has greatly strengthened people's understanding of the genetic characteristics and molecular mechanisms of rice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H6/46
CPCC07K14/415C12N15/8235C12N15/8261
Inventor 李双成李平高烽焱王世全邓其明郑爱萍朱军刘怀年王玲霞
Owner SICHUAN AGRI UNIV
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