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Application of osgbp3 gene in regulating rice plant height, grain shape and thousand-grain weight

A thousand-grain weight and rice technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve problems such as reducing the quality of rice, and achieve the effects of increasing thousand-grain weight, reducing grain length and promoting growth.

Active Publication Date: 2021-03-23
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, genes that regulate grain width often reduce rice quality while increasing yield, such as GW2, GW8, etc. Therefore, the discovery and application of new grain shape genes has important production and application value for increasing rice yield and improving rice quality

Method used

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  • Application of osgbp3 gene in regulating rice plant height, grain shape and thousand-grain weight
  • Application of osgbp3 gene in regulating rice plant height, grain shape and thousand-grain weight
  • Application of osgbp3 gene in regulating rice plant height, grain shape and thousand-grain weight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Cloning of Example 1 Gene OsGBP3

[0135] The DNA of rice variety Nipponbare was extracted (primer sequence: upstream primer 5′-GATCAATCAGAAGACACG-3′ and downstream primer 5′-CCAGATTAGGTACAGAACCT-3′) for polymerase chain reaction (PCR), and the obtained PCR product was sequenced to obtain the gene The gene sequence of OsGBP3 consists of 3484 bases, and the nucleotide sequence is shown in SEQ ID NO:1. PCR program: pre-denaturation at 94°C for 5 minutes; 35 cycles (denaturation at 94°C for 30 seconds; annealing at 55°C for 30 seconds; extension at 72°C for 4 minutes), and extension at 72°C for 10 minutes. Extract the RNA of rice variety Nipponbare leaves, reverse transcribe into cDNA, and use primers (primer sequence: upstream primer 5'-GGATCCCAGCGCCGCTCTTGTTCGA-3' and downstream primer 5'-TCTAGAGCAGAGCAACCTACAAAGC-3') to carry out polymerase chain reaction (PCR) , the size of the amplified product is 1137bp (including 65bp 5'untranslated region and 46bp 3'untranslated r...

Embodiment 2

[0137] The construction of embodiment 2 recombinant vector and the establishment of transformed agrobacterium

[0138] (1) The sequence containing the OsGBP3 gene CDS (SEQ ID NO: 2) amplified in Example 1 was double-digested with BamHI and Xba I, the target product was separated and recovered, and double-digested with BamHI and Xba I The pCAMBIA1301S vector was ligated with T4 ligase to form an overexpression vector. The above primers were synthesized by Shanghai Sangong, and the restriction enzymes BamH I, Xba I and T4 ligase were purchased from Takara Company.

[0139] (2) According to figure 1 Carry out the polymerase chain reaction (PCR) with the CDS sequence that embodiment 1 obtains with primer (primer sequence is: upstream primer 5'-AAAGAGCTCGGATCCTGGACCTCTCAAGGATACCAAC-3' and downstream primer 5'-AAAACTAGTGGTACCATGCTTCGCCCAGAAGGTCTTC-3') , a 269-base cDNA fragment of the gene OsGBP3 was isolated, the sequence of which was shown in SEQ ID NO:4. PCR program: pre-denat...

Embodiment 3

[0142] Example 3 Agrobacterium-mediated genetic transformation

[0143] (1) Induction: Shell the seeds of mature rice varieties (Zhonghua 11 and Nipponbare), and then treat them with 75% volume ratio of ethanol for 1 minute, and 0.15% concentration of mercury chloride (HgCl 2 ) Disinfect the surface of the seeds for 18 minutes; wash the seeds 4-5 times with sterilized water; place the seeds on the japonica rice induction medium; place the inoculated medium in a dark place for 4 weeks at a temperature of 25±1°C.

[0144] (2) Subculture: select bright yellow, compact and relatively dry embryogenic calli, put them on the japonica rice subculture medium and culture them in the dark for 2-3 weeks at a temperature of 25±1°C.

[0145] (3) Agrobacterium culture:

[0146] In the LA medium with kanamycin resistance (product of Shanghai Shenggong Company) selected (the preparation of LA medium can refer to J. Science Press, 2002) to pre-culture Agrobacterium strains TOX and FR for two ...

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Abstract

The invention provides an application of an OsGBP3 gene in regulating the rice plant height, grain shape and thousand kernel weight. The gene positively regulates the plant height and seed size; the gene is over-expressed, the plant growth is promoted, and not only is the plant height increased but also the grain length and grain width of seeds are increased so as to increase the thousand kernel weight. The expression of the gene is inhibited, the plant height is reduced and the grain length is reduced. Valuable gene resources are provided for crop breeding, and the rice OsGBP3 gene can be widely applied to hybrid breeding and hybrid seed production of crops.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to the application of OsGBP3 gene in regulating rice plant height, grain shape and thousand-grain weight. Background technique [0002] Rice is the main food crop for more than half of the world's population. Dwarf breeding and hybrid breeding have made rice production experience two leaps. However, with the deterioration of the natural ecological environment and the continuous reduction of arable land, rice production can be improved through traditional breeding methods. , it is becoming more and more difficult to meet the ever-increasing population, so increasing the yield per unit area of ​​rice has gradually become the goal of breeders, and the cultivation of ideal plant type materials is one of the ways to achieve high yield of rice per unit area. [0003] Plant height is the main determinant factor affecting rice plant type, and it is also an impor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 余四斌龚蓉
Owner HUAZHONG AGRI UNIV
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