Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rice grain type gene qGL6-2 and application thereof

A kind of grain type and rice technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of lower quality, difficult use, complicated operation, etc., and achieve the effect of easy operation

Active Publication Date: 2020-08-07
HUAZHONG AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the effect of GW2, qSW5 / GW5 and GW8 on the yield and appearance quality is the opposite effect, that is, the increase of yield will reduce the quality, and the improvement of yield and quality cannot be considered in the process of improving rice quality by using them
Although GS3 and qGL3.1 can be used to improve yield and quality at the same time, because the short-grain allele is dominant to the long-grain gene, it is relatively complicated to operate in rice breeding, especially in hybrid rice breeding. difficulty

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rice grain type gene qGL6-2 and application thereof
  • Rice grain type gene qGL6-2 and application thereof
  • Rice grain type gene qGL6-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The cloning of embodiment 1 gene qGL6-2

[0037] 1. Extract the genomic DNA of rice variety ACC10 (IRGC105491), use primers 5'-CCGATGAACGGTTTTGTA-3' and 5'-TCGGAGAGATGTGTCGAGCTCTT-3' to carry out polymerase chain reaction (PCR), and sequence the obtained PCR products to obtain rice The promoter sequence of the gene qGL6-2 consists of 2947 bases, and the nucleotide sequence shown is SEQ ID NO.1. The PCR program was as follows: pre-denaturation at 94°C for 5 minutes; 35 cycles (denaturation at 94°C for 30 seconds; annealing at 55°C for 30 seconds; extension at 72°C for 3 minutes), and extension at 72°C for 10 minutes.

[0038] 2. Extract the genomic DNA of rice variety ACC10 (IRGC105491), use primers 5'-AAGAGCTCGACACATCTCTCCGA-3' and 5'-TAAGCAGTAAGAACACG-3' to carry out polymerase chain reaction (PCR), and sequence the obtained PCR products to obtain rice The gene sequence of the gene qGL6-2 contains 6807 bases, and the nucleotide sequence shown is SEQ ID NO.2. The PCR ...

Embodiment 2

[0042] Example 2 Transgenic complementary qGL6-2 gene increases rice grain length and thousand-grain weight

[0043] 1. Construction of complementary vectors

[0044] According to the DNA sequence of the rice gene qGL6-2, see the promoter sequence of SEQ ID NO.1, respectively introduce the upstream and downstream primers on both sides of the Kpn I and BamH I restriction sites of the pC1301 vector, and design the primers as follows:

[0045] P0-F:5'-AAACGGGGTACCCCGATGAACGGTTTTGTA-3'

[0046] P0-R: 5'-AAACGCGGATCCTCGGAGAGATGTGTCGAGCTCTT-3'

[0047] Using the qGL6-2 promoter clone obtained in Example 1 above as a template, polymerase chain reaction (PCR) was performed with primers PO-F and PO-R, and the product size was 2973bp. The PCR program was as follows: pre-denaturation at 94°C for 5 minutes; 30 cycles (denaturation at 94°C for 30 seconds; annealing at 55°C for 30 seconds; extension at 72°C for 3 minutes), and extension at 72°C for 10 minutes. The qGL6-2 promoter fragmen...

Embodiment 3

[0073] Example 3 Application of rice gene qGL6-2 in improving grain shape and thousand-grain weight of rice variety ZS97

[0074] 1. Construction of near-isogenic lines

[0075] Taking ZS97 as the recipient parent and ACC10 as the donor parent, through crossing once, the recipient parent ZS97 was continuously backcrossed for 4 generations, combined with molecular marker-assisted selection, and finally obtained a near genetic background of ZS97 with qGL6-2 replaced by ACC10. The isogenic line was named NIL-ACC10. The genotype was detected by resequencing, and it was found that the whole genome only contained a 2Mb ACC10 import fragment at qGL6-2. For the genotype of NIL-ACC10, see Figure 6 .

[0076] 2. Expression identification

[0077] Underground parts (Root) and aboveground parts (Seeding) of NIL-ACC10 and ZS97 7-day-old seedlings, flag leaves, stems at the heading stage, ear parts with ear lengths of 0-2cm, 4cm, 8cm, 12cm, 16cm, and 20cm Tissues, total RNA was extrac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a rice grain type gene qGL6-2 and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO.2, and the CDS sequence of the gene is shown as SEQ ID NO.3. Theinvention also provides a rice grain type gene qGL6-2 and application thereof. The qGL6-2 gene complementary vector is transferred into indica rice ZS97 through agrobacterium tumefaciens mediated genetic transformation. A T0-generation transgenic plant then is obtained through selective culture, differentiation, rooting and seedling hardening; it is found that the seed length and thousand seed weight of a positive transgenic plant are remarkably larger than those of a negative transgenic plant. When the qGL6-2 gene is applied to improvement of grain shape, thousand seed weight, single plant yield and lodging resistance of rice varieties, an expected good effect is achieved, the rice varieties with longer grain length, increased thousand seed weight, increased yield and stable yield can becultivated, and the qGL6-2 gene has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a rice grain type gene qGL6-2 and its application. Background technique [0002] Rice is one of the most important crops, providing 21% of the global per capita calorie intake. In the case of gradually decreasing arable land, in order to meet the growing population's demand for food, it is of great significance to increase the unit yield of rice. Data show that rice yield per plant is mainly composed of three yield factors: effective panicle per plant, grain per panicle and thousand-grain weight. These yield traits are all quantitative traits (Quantitative trait loci, QTL), controlled by many genes with different effects . Grain shape has a great influence on appearance quality, and grain shape also has a great influence on rice yield. The national high-quality rice quality standard clearly stipulates that the first-class aspect ratio of indica ri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/113C12N15/82A01H5/10A01H6/46
CPCC07K14/415C12N15/8261
Inventor 余四斌王电文孙文强高大伟王记林孙强
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com