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Application of uch677 protein and its coding gene in regulating plant growth and development

An encoding gene, growth and development technology, which is applied in the application field of UCH677 protein and its encoding gene in regulating plant growth and development, can solve the problems of inability to meet the urgent needs of production and development, slow effect and long cycle, etc.

Active Publication Date: 2016-04-13
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For a long time, people have been using conventional breeding methods to select hybrid crops. These methods have a long cycle and slow results, and cannot meet the urgent needs of production development.

Method used

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  • Application of uch677 protein and its coding gene in regulating plant growth and development
  • Application of uch677 protein and its coding gene in regulating plant growth and development
  • Application of uch677 protein and its coding gene in regulating plant growth and development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Example 1. Obtaining and Identification of UCH677 Overexpression Transgenic Rice Plants

[0112] The UCH677 gene involved in this embodiment is derived from rice (Oryza.sativaL.), its cDNA sequence is as shown in sequence 2 in the sequence listing, and sequence 2 consists of 534 nucleotides; sequence 2 encodes the sequence 1 in the sequence listing The protein shown (UCH677 protein), sequence 1 consists of 177 amino acid residues.

[0113] 1. Construction of recombinant expression vector pCAM23A-UCH677ox

[0114] Design the following primer sequences according to sequence 2 in the sequence listing:

[0115] 23A677-F: 5'-CC TCTAGA ATGCTACGTTGTGGCGCA-3' (underlined is the recognition sequence of restriction site XbaI, followed by the 1-18th position of sequence 2);

[0116] 23A677-R: 5'-CC GTC GAC TCACTGCTTTGAAAGAGC-3' (underlined is the recognition sequence of the enzyme cutting site SalI, followed by the reverse complement of the 517-534th position of sequence 2)....

Embodiment 2

[0163] Example 2, Functional Identification of UCH677 Transgenic Rice Plants

[0164] Identify positive T with Example 1 1 The transgenic rice transgenic plants transformed into the recombinant expression vector pCAM23A-UCH677ox, the wild-type rice variety Zhonghua No. 11 transformed with the empty vector gene, and the control plants transformed into the pCAM23A empty vector obtained in Example 1 were used as experimental materials. Seeds of each experimental material were sown in petri dishes for germination (80-100 seeds were sown for each experimental material), and the seedlings after germination were transplanted in flowerpots to emerge, and then transferred to large fields in the suburbs of Beijing for growth. Carry out the analysis and identification of the following aspects. 10 strains were randomly selected for each genetic material for the experiment, and for the quantitative data, the average value of the 10 strains was taken as the result.

[0165] 1. Rice plant ...

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Abstract

The invention discloses application of protein UCH677 and a coding gene thereof in regulation and control of growth and development of plants. The application means application of protein, which is formed by an amino acid sequence represented by a sequence 1 in a sequence listing, or a coding gene thereof in regulation and control of growth and development, namely one of (1) to (10), of the plants: (1) seed weight, (2) seed volume, (3) seed grain length, (4) seed grain width, (5) seed grain thickness, (6) number of seeds per ear, (7) plant height, (8) plant leaf length, (9) plant leaf width and (10) plant ear length. Shown by experiments, during the development of paddy rice, through increasing the expression level of the protein UCH677 in the paddy rice, compared with wild-type rice seeds, rice seeds can have the following phenotypes that the weight and volume of the seeds are increased, and meanwhile, the number of the seeds per ear is increased; in addition, in the aspect of plant phenotype, the height of over-expressed plants is higher than that of the wild-type plants. According to the application, a foundation for finding out ideas and methods for more simply creating the high-yield traits of crops is laid.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and relates to the application of UCH677 protein and its encoding gene in regulating plant growth and development. Background technique [0002] Since the 1970s, the utilization of heterosis has made a significant contribution to rice production in my country. Facing the new and important demand of "high yield, high quality, high efficiency, safety, and ecology" put forward by my country's economic development for agricultural production, whether to further explore the application potential of heterosis has become a severe challenge for contemporary scientists. [0003] The formation of heterosis is based on the combination of two different parents. To further explore its potential on the basis of existing applications, in addition to strengthening the research on the formation mechanism of heterosis, it is also urgent to establish effective methods to create male sterility trait...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 王东辉白书农许智宏
Owner PEKING UNIV
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