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Method for quickly producing konjak tissue culture miniature potato in bottle

A production method and miniature tuber technology, which is applied in the field of crop cultivation, can solve the problems of low economic benefit, high production cost, and poor quality of planting yams, and achieve the effects of increasing the survival rate of seedlings, saving production costs, and improving tuber production efficiency

Active Publication Date: 2020-10-30
安康市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) The quality of seed taro is poor, and the reproduction coefficient is low, which cannot meet the needs of industrial development
(3) The cultivation technology is backward. At present, it is mainly planted in forest land with high altitude, which restricts the use of agricultural machinery, resulting in high production costs and low economic benefits.
However, the traditional transplanting of tissue culture seedlings is not only a complicated process, but also has a low survival rate, which seriously restricts the industrialized development of konjac tissue culture and rapid propagation.

Method used

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  • Method for quickly producing konjak tissue culture miniature potato in bottle
  • Method for quickly producing konjak tissue culture miniature potato in bottle
  • Method for quickly producing konjak tissue culture miniature potato in bottle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of rapid production method in the konjac tissue culture miniature potato bottle, comprises the following steps:

[0022] S1: In November, select well-growing konjac embryogenic callus and transfer to bud differentiation induction medium: MS+2.0mg / LKT+0.5mg / L6-BA+0.2mg / L2,4-D+0.1mg / LNAA, maintain indoor ambient temperature 25°C, humidity 50%, light intensity 3500lx, light time 8 / 16h;

[0023] Clustered buds differentiated in about 21 days, and seedlings grew out in about 39 days. The average number of buds differentiated was 13.7, and the average number of seedlings was 8.1;

[0024] S2: Transfer the clustered seedlings differentiated in S1 into rooting and strong seedling medium: 1 / 2MS+1.5

[0025] mg / LNAA+0.5mg / L2,4-D+20mg / L uniconazole, maintain the indoor ambient temperature at 25°C, humidity at 80%, light intensity at 6000lx, and light time at 8 / 16h;

[0026] The roots grow completely in about 16 days, the average number of roots is 11.8, the average root ...

Embodiment 2

[0035] A kind of rapid production method in the konjac tissue culture miniature potato bottle, comprises the following steps:

[0036] S1: In December, select konjac embryogenic calli with consistent growth and good growth and transfer them to bud differentiation induction medium: MS+3.5mg / LKT+1.0mg / L6-BA+0.4mg / L2,4-D+0.2 mg / LNAA, maintain the indoor ambient temperature at 28°C, humidity at 60%, light intensity at 5000lx, and light time at 8 / 16h;

[0037] Clustered buds can be differentiated in about 20 days, with an average number of differentiated buds of 14.7; about 35 days, seedlings can grow, with an average number of differentiated seedlings of 9.2;

[0038]S2: Transfer the clustered seedlings differentiated in S1 into rooting and strong seedling medium: 1 / 2MS+3.0 mg / LNAA+0.8mg / L2,4-D+40mg / L uniconazole; maintain the indoor temperature at 28°C, Humidity 60%, light intensity 5000lx, light time 8 / 16h;

[0039] The roots are fully differentiated in about 15 days, the aver...

Embodiment 3

[0051] A kind of rapid production method in the konjac tissue culture miniature potato bottle, comprises the following steps:

[0052] S1: In January, select konjac embryogenic calli with consistent growth and good growth and transfer them to bud differentiation induction medium: MS+4.0mg / LKT+1.0mg / L6-BA+0.5mg / L2,4-D+0.2 mg / LNAA. Maintain the indoor environment temperature at 26°C, humidity at 60%, light intensity at 5000lx, and light time at 8 / 16h;

[0053] Clustered buds can be differentiated in 23 days, with an average number of bud differentiations of 13.2, and seedlings will grow in 36 days, with an average number of differentiated seedlings of 9.4;

[0054] S2: Transfer the clustered seedlings differentiated in S1 into the rooting and strong seedling medium: 1 / 2MS+2.5 mg / LNAA+1.0mg / L2,4-D+35mg / L uniconazole, maintain the indoor temperature at 26°C, Humidity 80%, light intensity 5500lx, light time 8 / 16h;

[0055] The roots are fully differentiated in about 16 days, the...

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Abstract

The invention belongs to the technical field of crop cultivation, and particularly relates to a method for quickly producing konjak tissue culture miniature potato in a bottle. The method includes: transferring seedlings to a culture medium, and maintaining a temperature at 26 DEG C-30 DEG C, a humidity at 60%-80%, a light source as blue light, red light and red-blue combined light, a color ratioof a red-blue lamp as 5-10: 1, an irradiation time as 2-4 h / red 1-2 h / blue-red 5-6 h, an illumination intensity as 6000-7500 lx, an illumination time as 12 / 12 h, and a CO2 concentration as 5%-8%. According to the method, the tissue culture seedlings can grow new roots in 4-6 days, small seed potatoes can grow in 15-20 days, and 8-14 g seed potatoes can be produced in 40-55 days, with an average of6.4 potatoes per bottle. The method omits many links in a process of transplanting the konjac seedlings outside the bottle, saves production costs, and effectively improves production efficiency of original seeds of konjac tissue culture.

Description

technical field [0001] The invention belongs to the technical field of crop cultivation, in particular to a method for rapid production of konjac tissue culture miniature potatoes in a bottle. Background technique [0002] Konjac is a perennial herbaceous plant belonging to Araceae Konjac. Konjac contains a large amount of soluble dietary fiber - glucomannan, which is the seventh largest nutrient for the human body. It is one of the top ten health foods identified by the United Nations. Convenience, beauty, etc. have significant effects. Konjac is used as food after processing. It is a gel food with outstanding health effects and high fiber content. It can promote gastrointestinal peristalsis and help the body digest and absorb. Used in food, medicine, chemical and biological fields. [0003] However, along with the continuous expansion of the industry, some technical problems to be solved urgently also appeared thereupon. For example, (1) The occurrence of diseases is ve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 蔡阳光段龙飞覃剑锋陈国爱张百忍刘次鹏杨凉花王宗方
Owner 安康市农业科学研究院
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