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Method for backcross breeding by utilizing plant male sterile material and application

A technology of male sterility and backcross breeding, applied in the field of multi-line variety breeding, can solve the problems of increasing the probability of occurrence of target single plants, heavy breeding workload, and low efficiency

Pending Publication Date: 2020-10-27
江西省超级水稻研究发展中心
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to aim at the shortcomings of large workload and low efficiency in the cultivation of existing plant multi-line varieties, and propose a method and application of backcross breeding using plant male sterile materials. A male sterile line, which is applied to the backcross breeding process, can greatly reduce the labor cost of detasseling, and it is easy to increase the number of hybrids to increase the probability of the appearance of the target individual plant, thereby shortening the breeding process and improving efficiency

Method used

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  • Method for backcross breeding by utilizing plant male sterile material and application
  • Method for backcross breeding by utilizing plant male sterile material and application
  • Method for backcross breeding by utilizing plant male sterile material and application

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Embodiment 1

[0044] Example 1. Using TDR gene loss-of-function recessive male sterile materials for backcross breeding in rice

[0045] Rice is the most important crop in my country, and it is also a typical diploid plant. In the specific embodiments of the present invention, we use rice as the object of implementation to demonstrate the technical solutions of the present invention. Specifically, using the male sterility caused by the TDR gene function loss for backcross breeding, the OsNramp5 mutant gene was introduced into the background parent variety Tainan 11 (a tropical japonica rice variety) to create a low-cadmium Tainan 11 line. Specific steps are as follows:

[0046] 1. Transform the background parent Tainan 11 into a male sterile material

[0047] The TDR gene mutation Tainan 11 male sterile material was created by the CRISPR / Cas9 system-mediated gene knockout technology (for the experimental process and the reproduction of the male sterile material, please refer to the invention pate...

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Abstract

In order to improve the efficiency of backcross work required by cultivation of different strains in cultivation of multi-line varieties, the invention provides a method for backcross breeding by utilizing a plant male sterile material and an application. According to a technical scheme for introducing a nuclear recessive male sterile gene into a background parent of a multi-line variety and assisting backcross breeding, the workload of field artificial hybridization can be greatly reduced, so the scale of backcross groups is easily expanded; the working efficiency of backcross breeding is improved; and the breeding period is shortened. The method provided by the invention is helpful to promote cultivation of the multi-line varieties.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and relates to a method for backcrossing breeding using plant male sterile materials and its application in multi-line variety breeding. Background technique [0002] With the identification and cloning of a large number of favorable plant traits controlling genes (or genetic loci), the breeding application of these genes (or genetic loci) has become the main task of current breeding. Breeding and utilization of known genes (or genetic sites) is mainly carried out through molecular marker-assisted selection. The current mainstream approach is to aggregate a few favorable genes into a certain variety through molecular marker-assisted selection, and with favorable genes With the increase in the number, it becomes very difficult to aggregate them into one species. It is even more difficult to aggregate different genes located on the same chromosome into one species. But cultivating multi-line var...

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 龙起樟黄永兰万建林王会民芦明唐秀英朱雪晶
Owner 江西省超级水稻研究发展中心
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