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Method for tracking blood-entering components of five-ingredient capsule for warming, dredging and removing arthralgia and targeting quality marker

A technology for blood components and markers, which is used in measurement devices, material separation, and analysis of materials, etc.

Active Publication Date: 2020-10-23
FIRST AFFILIATED HOSPITAL OF ANHUI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the diversity and complexity of TCM ingredients, the screening and analysis of its active ingredients face many challenges

Method used

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  • Method for tracking blood-entering components of five-ingredient capsule for warming, dredging and removing arthralgia and targeting quality marker
  • Method for tracking blood-entering components of five-ingredient capsule for warming, dredging and removing arthralgia and targeting quality marker
  • Method for tracking blood-entering components of five-ingredient capsule for warming, dredging and removing arthralgia and targeting quality marker

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Embodiment Construction

[0052] see figure 1 As shown in the flow chart of the steps, the present invention first combined serum medicinal chemistry and UHPLC-QTOF-MS technology to track the blood components of Wuwei Wentong Chubi Capsules, and then targeted 15 quality markers from the blood components, and analyzed Wuwei Wentong Chubi Capsules. Make a scientific evaluation of the quality of Wentong Chubi Capsules.

[0053] 1. Combining serum medicinal chemistry and UHPLC-QTOF-MS technology to track Wuwei Wentong Chubi Capsules (WWCC, composed of Poria cocos, Guizhi, Epimedium, Turmeric and Scutellaria baicalensis) into the blood and target its quality markers method

[0054] (1) The compound WWCC was prepared according to the proportion of the prescription by using five traditional Chinese medicines of Poria cocos, Guizhi, Epimedium, Scutellaria turmeric and Scutellaria baicalensis, and the WWCC extract was made into freeze-dried powder, which was used for gavage of rats and later sample analysis. ...

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Abstract

The invention discloses a method for tracking blood-entering components of a five-ingredient capsule for warming dredging and removing arthralgia and targeting a quality marker. The method comprises the following steps: selecting an SPF-grade male SD rat, and injecting FCA into the skin of the right rear toe to replicate an adjuvant arthritis (AA) rat model; the WWCC treatment group performs intragastric administration on the twelfth day after FCA injection, and the intragastric administration is continuously performed for 12 days; collecting and treating a serum sample, and carrying out QTOF-MS analysis on the serum sample, 13 standard substances and the compound WWCC extract; recording retention time information of each standard substance, and summarizing cracking rules of different types of compounds through mass spectrum cracking fragment information analysis. According to the principles that the deviation between a measured value and a theoretical value is smaller than 10 ppm andthe isotope fitting degree is smaller than 1.0, a molecular formula corresponding to each spectral peak is obtained through a mass system element composition calculation tool; and finally, the compound WWCC in-blood component is identified by combining a standard fragment cracking rule and data, and a representative component is selected as a quality marker from the compound WWCC in-blood component.

Description

technical field [0001] The invention belongs to the technical field of identification of active components of traditional Chinese medicine compound prescriptions, and in particular relates to a method for tracking blood components of Wuwei Wentong Chubi capsules and targeting quality markers. Background technique [0002] For thousands of years, traditional Chinese medicine has been widely used in many countries in Asia, especially China, for the prevention and treatment of various diseases. Their therapeutic effects are attributed to a large number of bioactive components extracted from single herbs or compound herbs. However, these bioactive components have not been fully elucidated. At present, the quantitative analysis of the quality control of traditional Chinese medicine compound mostly uses chemical components, rather than potential bioactive components. Therefore, in quality evaluation, there is an urgent need to develop a method suitable for finding biologically a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679G01N30/8686
Inventor 姜辉刘健王斌黄开泉万磊张玉婷凡畅
Owner FIRST AFFILIATED HOSPITAL OF ANHUI UNIV OF CHINESE MEDICINE
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