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Wheat ear branching gene DNA sequence amplification method, branching gene and application

A DNA sequence and gene technology, applied in the DNA sequence amplification method of wheat ear branch gene, branch gene and application field, can solve the problem of no clear location of gene

Pending Publication Date: 2020-10-23
CHUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Genes related to wheat ear branching traits have not been clearly located

Method used

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  • Wheat ear branching gene DNA sequence amplification method, branching gene and application
  • Wheat ear branching gene DNA sequence amplification method, branching gene and application
  • Wheat ear branching gene DNA sequence amplification method, branching gene and application

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Embodiment 1

[0035] In order to achieve the above object, the technical scheme adopted in the present invention is:

[0036] The invention provides a kind of wheat ear branching gene DNA sequence amplification method: detailed method is as follows: 1 material

[0037] (1.1) Test material

[0038] Common wheat varieties "China Spring" and "Xiaobaimang" were selected from the Chinese wheat micro-core collection; "Branched wheat" is a branched panicle mutant of "Zhengmai 9023", and the experimental materials were all planted in the Huashan Wheat Experimental Base of Chuzhou University. At the beginning of April every year, the young leaves and young ear tissues of four wheat varieties were taken respectively when they were just heading. The ear shapes of the four wheat varieties were compared as figure 1 As indicated, leaf DNA and total RNA from panicle tissues of each variety were extracted, and the extracted genomic DNA and total RNA were stored at -20°C and -80°C, respectively.

[0039]...

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Abstract

The invention discloses a wheat ear branching gene DNA sequence amplification method, a branching gene and application. Wheat leaves of four varieties including branched wheat, non-branched wheat, Chinese Spring and Xiaobaimang are used as test materials, and tb1 gene full lengths are respectively amplified in the four kinds of wheat by adopting a homologous cloning method. The analysis on the tb1genes of four kinds of wheat shows that the full length of the coding region of the gene is 1059bp, 352 amino acids are coded in total, and the gene is confirmed to be a branching gene through verification and participates in the regulation and control of wheatear branching. The invention provides a basis for cultivating more excellent wheat varieties, enriching breeding resources and further cultivating new crop varieties with excellent agronomic traits, high yield and lodging resistance, and also provides a better scientific basis for molecular genetic breeding, trait improvement and germplasm innovation of crops.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for amplifying the DNA sequence of a wheat ear branching gene, a branching gene and an application thereof. Background technique [0002] So far, wheat is a kind of food crop that is widely planted in most regions of the world, and provides most of the energy consumption basis for all human beings. Therefore, increasing wheat production is the basis for stabilizing the global economy, and it is also the basis for the sustainable health of the national economy. important goal of development. The yield of wheat is closely related to the fruiting part of wheat. Studies have shown that among the three elements of wheat yield, the number of wheat ears plays a major role. Therefore, the study of wheat ear shape development is not only beneficial to people's theoretical understanding of wheat ear development, but also beneficial to high-yield breeding of wheat. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6895C12N15/10C12N15/11C12N15/29C07K14/415
CPCC12Q1/6806C12Q1/6895C07K14/415C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 蔡华赵荣赵维萍
Owner CHUZHOU UNIV
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