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Determination method of microcarrier detection inhibitor

An assay method and microcarrier technology, applied in the field of phytopathology, can solve the problems of easy to miss, large sample volume, omission of inhibitory active substances, etc.

Active Publication Date: 2020-10-23
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This assay technical scheme often requires a relatively large sample volume of the sample to be tested. When the sample volume is too small, it is often impossible to detect, and it is easy to miss or omit inhibitory active substances with small content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Apply a kind of assay method of microcarrier detection inhibitor of the present invention, measure the inhibitory effect of medicine 25% benzazine to the spore germination of Fusarium wilt bacteria strain Fo-4, carry out operation as follows:

[0037] 1. Preparation of qualified microcarriers

[0038] 1) Sterilization of the concave glass slides: sterilize the clean concave slides at high temperature for later use.

[0039]2) Arrangement of the concave glass slides: Prepare the moisturizing device capable of holding the moisturizing material and the concave glass slides in advance, open the cover of the moisturizing device on the aseptic workbench, and place the sterilized concave glass slides of operation 1) stably on the Moisturizing material face.

[0040] 3) Melting of the agar medium: heat and melt the agar medium contained in a triangular flask suitable for germination of pathogenic bacteria spores.

[0041] 4) Disposal of the culture medium at 80°C: preheat an ...

Embodiment 2

[0054] Apply a kind of assay method of microcarrier detection inhibitor of the present invention, measure the inhibitory effect of drug 25% propiconazole to the spore germination of rice false smut bacterial strain Uv-111, operate and implement by the step 1 to step 5 of embodiment 1, different Yes, the drug sample loaded in step 2 is a 500-fold dilution of 25% propiconazole, and the spores loaded in step 3 are at a concentration of 10 6 spore / ml of the rice smut bacteria strain Uv-111 spore liquid, the cultivation time of step 4 is 48 hours.

[0055] Results The germination rate of spores treated with sterile water blank control was 94.00%, while the germination rate of 25% propiconazole treatment was 0; the inhibitory effect of propiconazole samples on the spores of rice false smut was 100%.

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Abstract

The invention relates to a phytopathology technology, and particularly discloses a determination method. 25 [mu]l of an agar culture medium microcarrier is prepared by adopting a concave slide, a drugsample and pathogenic bacteria spores are loaded on the microcarrier, and the inhibition effect of the drug on spore germination is detected. The determination method comprises the following steps: 1) preparing a qualified microcarrier; 2) loading the sample to be measured into the microcarrier; 3) dropping the pathogenic bacteria spores; 4) carrying out spore germination culture; and 5) observing results. The method has the advantages that 1) a plurality of samples can be tested on one plate, the miniaturization of a test system is realized; and 2) one test unit only consumes 10 microlitersof a sample solution, so that the micro-quantification of a detection sample is realized.

Description

technical field [0001] The invention relates to plant pathology technology, in particular to a method for measuring inhibitors detected by microcarriers. [0002] technical background [0003] At present and for a long time to come, pesticide control is still the main means of plant disease control. Exploring and excavating biological source pesticides is an important direction of pesticide control, and the early exploration and excavation of biological source pesticides often requires collecting or collecting substances that have inhibitory effects on plant pathogenic bacteria from nature, or isolating them from organisms or biological products that have inhibitory effects active ingredients. Obviously, efficient and feasible inhibitor identification and detection methods will help improve the effectiveness and efficiency of biogenic pesticide exploration and mining. The current common method for identifying the effect of inhibitors on pathogenic bacteria includes the assa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 王忠文张君成张正淳
Owner GUANGXI UNIV
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