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Application of WCR1 gene in regulating core-white rate or taste quality of rice

A food quality and gene technology, applied in the field of rice breeding, can solve the problems of ineffective application, no transgene, and lack of functional molecular markers.

Active Publication Date: 2020-10-23
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In reported studies, although multiple quantitative trait loci (QTL) associated with chalkiness have been identified, involving multiple chromosomes in the rice genome, there are basically no transgenes to verify the effects of these QTLs, and there is a lack of functional Molecular markers, so it cannot be effectively used in production practice

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  • Application of WCR1 gene in regulating core-white rate or taste quality of rice
  • Application of WCR1 gene in regulating core-white rate or taste quality of rice
  • Application of WCR1 gene in regulating core-white rate or taste quality of rice

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Embodiment Construction

[0034] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0035] 1. Material selection and establishment of recombinant inbred line (RIL) population

[0036] The high chalkiness japonica rice variety Hokuri 130 (BL130) and the low chalkiness indica rice variety Jin 23B (J23B) were selected for crossing to obtain F1, and the progeny were continuously selfed, and 184 F6 plants were obtained according to the method of "single seed transmission" as RIL group.

[0037] 2. Phenotype identification and genetic map construction

[0038] Using the SSR method to identify the genotype, screen the polymorphic SSR primers, and obtain 190 pairs of polymorphic SSR markers and 2 pairs of InDel markers. A genetic linkage map was constructed with RM series primers, and gaps were filled with...

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Abstract

The invention relates to a new multi-effect core-white-related gene WCR1. A transgenic material is constructed for verification, and finally a functional variation site is found and a corresponding molecular marker is developed, so that an effective theoretical basis and technical support are provided for improvement of chalkiness of rice. The WCR1 gene is used, through genetic manipulation, the core white rate can be reduced by about 20%, the head rice rate can be increased by about 10%, certain positive influences are also achieved on the yield and the taste value of a single plant, and theinfluences are rarely seen in production and application. The invention provides the molecular marker for low core white rate. The detection of the molecular marker can quickly identify a homozygous genotype single plant with low core white rate in the seedling stage, and timely eliminate single plants with high core white rate, so that the production cost is saved, the selection efficiency is greatly improved, and the breeding period of rice varieties is greatly shortened.

Description

technical field [0001] The present invention relates to the field of rice breeding, more particularly, relates to the application of WCR1 gene in regulating rice whiteness rate or eating quality. Background technique [0002] Rice (Oryza sativa L.), as one of the three major food crops in the world, provides food sources for half of the population on the planet, and provides up to 76% of the caloric intake for people in Southeast Asia (Sasaki et al., 2000; Wing et al., 2018). With the improvement of living standards, people pay more and more attention to the quality of rice. At the beginning of the 21st century, scientists put forward the concept of "green super rice", which regards high quality as an important goal of rice breeding. Rice chalkiness refers to the white opaque part of the endosperm, which is an unwelcome appearance quality trait for consumers and the market, and will also have a certain negative impact on the processing quality and cooking and eating qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12Q1/6895A01H1/02A01H1/04A01H5/10A01H6/46
CPCC07K14/415C12N15/8218C12N15/8261C12Q1/6895A01H1/02A01H1/04C12Q2600/13C12Q2600/158
Inventor 何予卿吴边周浩夏朵
Owner HUAZHONG AGRI UNIV
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