Quantitative isobaric oxygen removing device and method of anaerobic bottle for biological culture

An anaerobic bottle and equal pressure technology, which is applied in the fields of biology and analytical chemistry, can solve problems such as the inability to confirm the oxygen concentration and gas pressure of anaerobic bottles, and the inability to meet the requirements of quantitative deoxygenation

Active Publication Date: 2020-10-23
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the goal of the above deoxygenation methods is to remove all the oxygen in the anaerobic bottle, which cannot meet the requirements of quantitative deoxygenation to adapt to anaerobic culture or controlled atmosphere culture with a certain concentration of oxygen.
On the other hand, the above-mentioned culture method cannot confirm the oxygen concentration and gas pressure in the anaerobic bottle after deoxygenation is completed.

Method used

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  • Quantitative isobaric oxygen removing device and method of anaerobic bottle for biological culture
  • Quantitative isobaric oxygen removing device and method of anaerobic bottle for biological culture
  • Quantitative isobaric oxygen removing device and method of anaerobic bottle for biological culture

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Embodiment 1

[0025] Please refer to figure 1 , the embodiment of the present invention provides a kind of anaerobic bottle quantitative isobaric deaeration device that is used for biological culture, comprises the anaerobic bottle 1 that tool plug seals, headspace bottle 2 and water container 3; Said anaerobic bottle 1. The headspace bottle 2 and the water container 3 are connected by a vent tube 4 to form an airtight device; the vent tube 4 penetrates into the sealed anaerobic bottle 1 and the headspace bottle 2 respectively through a needle; the gas source is connected with the anaerobic bottle Part of the ventilation pipe 4 of 1 is the air inlet pipe 41, and the part of the ventilation pipe 4 connected with the headspace bottle 2 and the water container 3 is the air outlet pipe 42. The device uses the anaerobic bottle 1 to communicate with the headspace bottle 2 and the water container 3, and can realize the indirect determination of the oxygen concentration value of the anaerobic bottl...

Embodiment 2

[0062] Using the device and method of Embodiment 1 of the present invention to carry out quantitative isobaric removal of oxygen in the anaerobic bottle used for biological cultivation, comprising the following steps:

[0063] (1) V of anaerobic bottle 1 a They are 313.0, 160.0, 737.0, 1405.0 and 0mL respectively (a total of five groups of experiments, 0mL group is not connected to the anaerobic bottle), and the corresponding volume of the culture solution to be added V m 50, 20, 100, 100 and 0 mL respectively (a total of five groups of experiments), the volume of the headspace bottle 2 is 21.6 mL, which is substituted into the formula (1), and the target oxygen concentration in the anaerobic bottle 1 is calculated to be 13.5, 7.3, 4.7, 18.5, 3.2% (a total of five groups of experiments) required ventilation time were 10, 20, 150, 10, 8s (a total of five groups of experiments);

[0064] (2) Connect the quantitative isobaric deoxygenation device, feed nitrogen into the quantita...

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Abstract

The invention discloses a quantitative isobaric oxygen removing device and method of an anaerobic bottle. A closed anaerobic bottle of a certain volume is connected into two gas paths of inlet and outlet by adoption of a breather pipe; one end of a gas inlet pipe is connected with a stabilized gas source; one end of a gas outlet pipe is pierced into a closed headspace bottle; and the gas outlet pipe of the headspace bottle is inserted to be below the liquid surface in a container filled with water of a certain volume. According to the volume of a sample bottle, the volume of culture solution to be added and the oxygen removing target concentration requirement, the target ventilation time is calculated by utilization of a quantitative prediction model; after the stabilized gas source is opened to continuously ventilate to the set time, the gas inlet pipe is pulled out instantly; when the gas outlet pipe does not bubble any longer, the gas outlet pipe is pulled out instantly; at this time, the pressure in the anaerobic bottle is the same as the atmospheric pressure; furthermore, the oxygen concentration can be obtained through a determination result of the oxygen concentration in theheadspace bottle through a headspace gas chromatography; quantitative isobaric oxygen removing of the anaerobic bottle is realized; and simultaneously, the oxygen concentration of the anaerobic bottle also can be obtained by calculation through the prediction model according to the volume and the ventilation time of the anaerobic bottle.

Description

technical field [0001] The invention belongs to the technical field of biology and analytical chemistry, and in particular relates to an anaerobic bottle quantitative isobaric oxygen removal device and method for biological cultivation. Background technique [0002] Anaerobic microbial infection accounts for an increasing proportion of clinical infections in various departments, so it has also received more and more attention. Anaerobic bacteria are an important part of the normal flora in the human body, so anaerobic bacterial infections are mostly endogenous and frequent. Anaerobic culture or controlled atmosphere culture is an important means to study the growth and pathogenicity of anaerobic or facultative anaerobic microorganisms, and it is also a routine method for the isolation and identification of pathogenic anaerobic bacteria in clinical practice. One of the key steps of anaerobic culture or controlled atmosphere culture is to create a quantitative isobaric gas en...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/24C12M1/04G01N30/02G01N30/86
CPCC12M23/08C12M23/38C12M29/06C12M29/22C12M41/34G01N30/02G01N30/8675
Inventor 张春云郭明全李梦辉
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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