Medicine for resisting atherosclerotic inflammation and anti-inflammatory effect detection method

Abstract

The invention belongs to the technical field of atherosclerotic targeted drugs, and discloses a medicine for resisting atherosclerotic inflammation and an anti-inflammatory effect detection method, and the medicine for resisting atherosclerotic inflammation is artesunate. The method for detecting the anti-inflammatory effect of the medicine for resisting atherosclerotic inflammation comprises thefollowing steps: performing an in-vitro experiment of using ox-LDL to induce VSMC phenotype to be converted into inflammatory phenotype cells; performing an in vivo experiment of using ox-LDL to induce VSMC phenotype to be converted into inflammatory phenotype cells. According to the invention, a molecular biology method is adopted, and the artesunate and target protein NLRP3 binding target is comprehensively investigated through a cell model and an animal model for inhibiting ox-LDL from inducing phenotypic transformation of VSMCs inflammatory cells by artesunate; and the anti-inflammatory effect and the molecular mechanism of artesunate in AS vascular inflammatory injury are illustrated by inhibiting the anti-inflammatory effect and the molecular mechanism of ox-LDL induced VSMCs inflammatory cell phenotypic transformation.

Description

technical field [0001] The invention belongs to the technical field of atherosclerosis-targeted drugs, and in particular relates to a drug for anti-atherosclerotic inflammation and a method for detecting anti-inflammatory effects. Background technique [0002] Atherosclerosis (AS) is a chronic inflammatory response disease of the vessel wall, and the inflammatory response runs through its occurrence, development and prognosis. The pathogenesis that has been widely recognized is: the theory of inflammation based on the theory of "lipid infiltration and injury response". It is believed that AS is the accumulation of lipids in the inner wall of large and medium-sized blood vessels, recruiting monocyte-macrophages to invade and accumulate in blood vessels, releasing more inflammatory factors, and promoting the phenotype of vascular smooth muscle cells (VSMCs) located in the middle layer of blood vessels. Transformation, the transformed VSMCs have abnormal biological functions o...

AI Technical Summary

Problems solved by technology

[0011] Through the above analysis, the problems and defects of the existing technology are: inflammation is one of the main pathogenic factors of atherosclerosis AS, and the inflammatory damage of blood vessels is related to the transformation of VSMCs in vascular smooth muscle cells. At present, there is still a lack of effective anti-inflammatory drugs

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