Preparation method and application of apoferritin-based encapsulated Ru (bpy) 3<2+> immunosensor
A technology of apoferritin and immunosensor, which is applied in the field of new nanomaterials and biosensing, can solve the problems of signal stability, poor reproducibility, poor biocompatibility, adverse effects on accuracy, etc., and achieve high sensitivity , reproducible, easy to prepare effects
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Embodiment 1
[0024] Embodiment 1. A kind of encapsulation Ru(bpy) based on apoferritin 3 2+ The preparation method and application of the immunosensor is characterized in that it comprises the following steps:
[0025] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;
[0026] (2) Drop-coat 6 μL of CYFRA 21-1 antibody-labeled Ru(bpy) at a concentration of 5 mg / mL on the surface of the glassy carbon electrode 3 2+ The @apoferritin solution was used as the sensing substrate, which was dried at 37 °C;
[0027] (3) Add 3 μL bovine serum albumin solution with a mass fraction of 1% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;
[0028] (4) Add 6 μL of CYFRA 21-1 antigen solution with a concentration of 10 μg / mL or an unknown concentration, incuba...
Embodiment 2
[0029] Example 2.1. A kind of encapsulation Ru(bpy) based on apoferritin 3 2+ The preparation method and application of the immunosensor is characterized in that it comprises the following steps:
[0030] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;
[0031](2) Drop-coat 6 μL of CYFRA 21-1 antibody-labeled Ru(bpy) at a concentration of 10 mg / mL on the surface of the glassy carbon electrode 3 2+ The @apoferritin solution was used as the sensing substrate, which was dried at 37 °C;
[0032] (3) Add 3 μL of bovine serum albumin solution with a mass fraction of 2% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;
[0033] (4) Add 6 μL of CYFRA 21-1 antigen solution with a concentration of 10 μg / mL or an unknown concentration, incu...
Embodiment 3
[0034] Example 3.1. A kind of encapsulation Ru(bpy) based on apoferritin 3 2+ The preparation method and application of the immunosensor is characterized in that it comprises the following steps:
[0035] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;
[0036] (2) Drop-coat 6 μL of CYFRA 21-1 antibody-labeled Ru(bpy) at a concentration of 15 mg / mL on the surface of the glassy carbon electrode 3 2+ The @apoferritin solution was used as the sensing substrate, which was dried at 37 °C;
[0037] (3) Add 3 μL bovine serum albumin solution with a mass fraction of 3% dropwise to block the non-specific active sites on the electrode surface, rinse the electrode surface with pH 7.4 phosphate buffer solution PBS, and place it at 4 °C C to dry;
[0038] (4) Add 6 μL of CYFRA 21-1 antigen solution with a concentration of 10 μg / mL or an unknown concentration, incuba...
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