New application of isoastragaloside II in preparation of tumor immune treatment drugs

A technology of isoastragaloside and astragaloside, which is applied in the field of isoastragaloside II in the preparation of tumor immunotherapy drugs, and can solve problems such as insufficient research

Inactive Publication Date: 2020-10-13
天津琪琛科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether isastragaloside Ⅱ can become a compound that plays a major role in tumor immunotherapy has not been fully studied.

Method used

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  • New application of isoastragaloside II in preparation of tumor immune treatment drugs
  • New application of isoastragaloside II in preparation of tumor immune treatment drugs
  • New application of isoastragaloside II in preparation of tumor immune treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Isastragaloside II enhances CD8 + T cell immune response

[0036] Experimental materials: 8-week-old male C57 mice (purchased from Victoria Lihua Company), anti-mouse CD3, anti-mouse CD28 (BD), IL-2 (R&D), PE-anti-mouse CD8a; APC-anti- mouse IFN-γ; Per CP anti-mouseperforin (BD), CFSE dye, propylene glycol methyl ether acetate (PMA); ionomycin (purchased from sigma), fetal bovine serum, 1640 medium, DMEM culture base (Gibco), isoastragaloside Ⅱ (sigma);

[0037] Test content:

[0038] 1. Preparation of spleen single-cell suspension: kill the mouse, take the spleen, crush the tissue with a 200-mesh filter, collect the cells in 1640 culture medium, centrifuge at 1400 r / min to discard the supernatant, use the erythrocyte lysate to remove the red blood cells, and collect the lysate Then add 10% fetal bovine serum 1640 culture medium to the cells for later use;

[0039] 2. Use anti-mouse CD3 (10μg / mL) to coat the plate overnight;

[0040] 3. Use anti-mouse CD...

Embodiment 2

[0046] Example 2: Effect of isoastragalosides on the killing effect of CTL cells

[0047] Experimental materials: 8-week-old male C57 mice (purchased from Victoria Lihua Company), anti-mouse CD3; anti-mouse CD28 (BD), IL-2 (R&D), PE-anti-mouse CD8a; APC-anti- mouse IFN-γ; PerCP anti-mouseperforin (BD), CFSE dye; propylene glycol methyl ether acetate (PMA); ionomycin (purchased from sigma), fetal bovine serum; 1640 medium, DMEM medium (Gibco), isoastragaloside II (sigma);

[0048] Test content

[0049] 1. Preparation of spleen single-cell suspension: kill the mouse, take the spleen, crush the tissue with a 200-mesh filter, collect the cells in 1640 culture medium, centrifuge at 1400 r / min to discard the supernatant, use red blood cell lysate to remove red blood cells, and collect and lyse Afterwards, the cells were added with 10% fetal bovine serum 1640 culture medium for later use.

[0050] 2. Use anti-mouse CD3 (10μg / mL) to coat the plate overnight

[0051] 3. Use anti-mo...

Embodiment 3

[0057] Example 3: Isastragaloside II regulates the killing effect of CTL

[0058] Experimental materials: 8-week-old male C57 mice (purchased from Victoria Lihua Company), anti-mouse CD3; anti-mouse CD28 (BD), IL-2 (R&D), PE-anti-mouse CD 8a; APC-anti -mouse IFN-γ; PerCP anti-mouseperforin (BD), CFSE dye; propylene glycol methyl ether acetate (PMA); ionomycin (purchased from sigma), fetal bovine serum; 1640 medium; DMEM culture Gibco (Gibco), Isastragaloside II (sigma)

[0059] Test content

[0060] 1. Preparation of spleen single-cell suspension: kill the mouse, take the spleen, crush the tissue with a 200-mesh filter, collect the cells in 1640 culture medium, centrifuge at 1400 r / min to discard the supernatant, use red blood cell lysate to remove red blood cells, and collect and lyse Then add 10% fetal bovine serum 1640 culture medium to the cells for later use;

[0061] 2. Use anti-mouse CD3 (10μg / mL) to coat the plate overnight;

[0062] 3. Use anti-mouse CD28 (1μg / mL)...

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PUM

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Abstract

The invention belongs to the technical field of biology, and discloses a new application of isoastragaloside II in preparation of tumor immune treatment drugs. The embodiment proves that the isoastragaloside II promotes CD8 + T cells to be differentiated into CTL cells, the characteristic of killing tumor cells is achieved, and the isoastragaloside II does not have cytotoxicity, so that the isoastragaloside II can be applied to preparation of tumor immune treatment drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a new application of isoastragaloside II, in particular to a new application of isoastragaloside II in the preparation of tumor immunotherapy drugs. Background technique [0002] In recent years, in the field of tumor treatment research, it has become a new trend to use the autoimmune system to resist tumors. By regulating the immune system, inhibiting and killing tumor cells has become an immunotherapy for tumors. Tumor immunotherapy is currently the most promising tumor treatment and research direction, and has achieved significant breakthroughs in areas such as immune response. Natural small molecular compounds in traditional Chinese medicine and natural compounds have broad prospects because of their small molecular weight, easy synthesis and administration. [0003] Astragaloside is a natural active substance that has a significant health care effect on human health, and has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P37/04
CPCA61K31/7048A61P35/00A61P37/04
Inventor 宋冰冰
Owner 天津琪琛科技有限公司
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