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Nano-enzyme bactericide based on bimetal organic framework as well as preparation method and application of nano-enzyme bactericide

An organic framework, bimetal technology, applied in the field of nano-enzyme bactericide and its preparation, can solve the problems of low stability, poor catalytic effect, inability to achieve sterilization effect, etc., and achieves inhibition of bacterial growth, low cost, enhanced electronic The effect of passing

Active Publication Date: 2020-10-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of conventional MOFs materials is relatively loose, accompanied by the release of metal ions in aqueous solution, it still has the disadvantages of low stability and poor catalytic effect, and cannot achieve effective sterilization effect.

Method used

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  • Nano-enzyme bactericide based on bimetal organic framework as well as preparation method and application of nano-enzyme bactericide
  • Nano-enzyme bactericide based on bimetal organic framework as well as preparation method and application of nano-enzyme bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of application of nano-enzyme bactericide based on double metal organic framework in inhibiting food-borne pathogenic bacteria, its steps are as follows:

[0042] (1) Dissolve 0.5mmol of cerium nitrate, 0.5mmol of copper nitrate and 1mmol of pyromellitic acid into 25mL of ethanol at the same time, mix well and place the solution on a magnetic stirrer (speed 1200rpm), and mix and react at room temperature for 2.5h ; After the reaction, the product was obtained by centrifugation, washed 5 times with ethanol, and then vacuum-dried at 60° C. for 12 hours to obtain a bimetallic organic framework;

[0043] (2) Place the bimetallic organic framework described in step (1) in a tube furnace (temperature 300° C.), and then calcinate it for 2.5 hours in an air atmosphere to obtain a nanozyme fungicide, which is stored in a sealed container at room temperature ;

[0044](3) Take a small amount of Escherichia coli (ATCC700728) strains on the LB solid medium, then place the L...

Embodiment 2

[0049] A kind of application of nano-enzyme bactericide based on double metal organic framework in inhibiting food-borne pathogenic bacteria, its steps are as follows:

[0050] (1) Dissolve 0.5mmol of cerium nitrate, 0.5mmol of copper nitrate and 1mmol of pyromellitic acid into 25mL of ethanol at the same time, mix well and place the solution on a magnetic stirrer (speed 1200rpm), and mix and react at room temperature for 2.5h ; After the reaction, the product was obtained by centrifugation, washed 5 times with ethanol, and then vacuum-dried at 60° C. for 12 hours to obtain a bimetallic organic framework;

[0051] (2) Place the bimetallic organic framework described in step (1) in a tube furnace (temperature 300° C.), and then calcinate it for 2.5 hours in an air atmosphere to obtain a nanozyme fungicide, which is stored in a sealed container at room temperature ;

[0052] (3) Take a small amount of Staphylococcus aureus (ATCC6538) strains on the LB solid medium, and then cul...

Embodiment 3

[0057] A kind of application of nano-enzyme bactericide based on double metal organic framework in inhibiting food-borne pathogenic bacteria, its steps are as follows:

[0058] (1) Dissolve 0.5mmol of cerium nitrate, 0.5mmol of copper nitrate and 1mmol of pyromellitic acid into 25mL of ethanol at the same time, mix well and place the solution on a magnetic stirrer (speed 1200rpm), and mix and react at room temperature for 2.5h ; After the reaction, the product was obtained by centrifugation, washed 5 times with ethanol, and then vacuum-dried at 60° C. for 12 hours to obtain a bimetallic organic framework;

[0059] (2) Place the bimetallic organic framework described in step (1) in a tube furnace (temperature 300° C.), and then calcinate it for 2.5 hours in an air atmosphere to obtain a nanozyme fungicide, which is stored in a sealed container at room temperature ;

[0060] (3) Take a small amount of Salmonella (ATCC14028) strains and streak on the LB solid medium, then place ...

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Abstract

The invention discloses a nano-enzyme bactericide based on a bimetallic organic framework as well as a preparation method and application of the nano-enzyme bactericide. The preparation method comprises the following steps of: (1) by taking a solution as a reaction medium, reacting cerium nitrate, copper nitrate and pyromellitic acid at room temperature to obtain a bimetal organic framework; and (2) calcining the bimetal organic framework in an air atmosphere at 200-400 DEG C for 1.5-3.5 hours to obtain the nano-enzyme bactericide based on the bimetal organic framework, which is used for inhibiting food-borne pathogenic bacteria. The nano-enzyme bactericide disclosed by the invention is a two-dimensional flaky quasi-MOFs material derived from the bimetal organic framework through low-temperature oxidation; the ano-enzyme bactericide has the biological enzyme-like catalytic activity, maintains the structural characteristics of the MOFs through the low-temperature oxidation, has improvedstability, enhances the electron transfer between metal sites, and greatly enhances the catalytic effect of nano-enzyme through the combination of the synergistic catalytic effect of double metals soas to achieve an efficient sterilization purpose.

Description

technical field [0001] The invention belongs to the field of food sterilization, and in particular relates to a nano-enzyme bactericide based on a bimetallic organic framework, a preparation method and application thereof. Background technique [0002] Foodborne pathogenic infections have become a worldwide healthcare problem due to high morbidity and mortality. Food-borne pathogenic bacteria often exist in nutrient-rich foods such as bread, leftovers, and milk. If the human body eats or becomes infected by mistake, it usually causes food poisoning, skin ulcers, and decreased immune system function. In severe cases, it may even cause meningeal inflammation and shock. Foodborne pathogenic infections have become one of the greatest threats to global public health, afflicting millions of people each year while causing serious economic losses. [0003] Many traditional and effective sterilization methods have been widely used in the suppression of foodborne pathogens, such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/22A01N59/00A01P1/00
CPCA01N59/00B01J31/1691B01J2531/0219B01J2531/16B01J2531/38
Inventor 孙大文黄伦杰周希艺蒲洪彬
Owner SOUTH CHINA UNIV OF TECH
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