Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Characteristic miRNA expression profile combination and early prediction method for renal clear cell carcinoma

A technology of renal clear cell carcinoma and prediction method, which is applied in the field of characteristic miRNA expression profile combination and early prediction of renal clear cell carcinoma, which can solve problems such as difficulty in defining critical values

Pending Publication Date: 2020-10-02
ANHUI MEDICAL COLLEGE
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the overlapping expression distribution of individual miRNAs in tumors and normal populations, it is difficult to define the cut-off value for early prediction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Characteristic miRNA expression profile combination and early prediction method for renal clear cell carcinoma
  • Characteristic miRNA expression profile combination and early prediction method for renal clear cell carcinoma
  • Characteristic miRNA expression profile combination and early prediction method for renal clear cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] A method for early prediction of clear cell renal cell carcinoma based on a combination of characteristic miRNA expression profiles, comprising the following steps:

[0118] Step 1. Obtain the characteristic miRNAs that are stably and differentially expressed in patients with early-stage clear cell renal cell carcinoma. For details, see figure 1 .

[0119] Step 1.1. Download the tumor tissue and paracancerous tissue transcriptome data and clinical data of clear cell renal cell carcinoma patients from the Genomic Data Commons Data Portal database, and obtain the read counts of the tumor tissue gene expression profiles of clear cell renal cell carcinoma patients, and perform logarithmic transformation.

[0120] Step 1.2: Select miRNAs with a certain expression abundance, that is, the read counts of miRNAs in all samples are greater than or equal to 10, see formula (1) for details.

[0121] Step 1.3: Select patients with clear cell renal cell carcinoma whose disease stage...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a characteristic miRNA expression profile combination and an early prediction method for renal clear cell carcinoma. The miRNA comprises hsa-let-7g, hsa-mir-10a, hsa-mir-10b, hsa-mir-125a, hsa-mir-21, hsa-mir-30b, hsa-mir-30c-1, hsa-mir-30c-2 and hsa-mir-532, and the nucleotide probe sequence is shown as SEQ ID NO. 1-9. The evaluation of the early risk of renal clear cell carcinoma based on the miRNA expression profile combination characteristics has high accuracy and accuracy (the area AUC under the ROC curve = 0.992). The early risk of renal clear cell carcinoma can be calculated by a support vector machine model only by acquiring the relative expression quantity of the nine miRNAs.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and in particular relates to a combination of characteristic miRNA expression profiles and an early prediction method for renal clear cell carcinoma. Background technique [0002] Kidney renal clear cell carcinoma accounts for about 70%-80% of renal cell carcinomas. Clear cell renal cell carcinoma is often asymptomatic in the early stage, or only has systemic symptoms such as fever and fatigue, so early diagnosis is difficult. Global Burden of Disease (GBD) data shows that in 2017, the number of people suffering from kidney cancer worldwide exceeded 2.1 million, of which about 270,000 were in China. In 2017, the number of deaths from kidney cancer worldwide was about 140,000, accounting for 0.25% of the total number of deaths. The number of patients who died in China in 2017 was about 17,000, accounting for 0.16% of the total number of deaths. The statistical results show that from 1...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886G16B25/10G16B35/00G16B5/00G16B40/00G16H50/20
CPCC12Q1/6886G16B25/10G16B35/00G16B5/00G16B40/00G16H50/20C12Q2600/158C12Q2600/178C12Q2600/112
Inventor 刘玮亓飞孙婷婷刘大海李文兴
Owner ANHUI MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com