Combination of compound acidifier and plant extract for feed
A compound acidifier and plant extract technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of less research on the application of active substances, improve intestinal health, reduce the ratio of consumption to weight gain, inhibit the intestinal The effect of pathogenic bacteria
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Embodiment 1
[0020] In order to evaluate the antibacterial effect of compound acidifier and plant extract combination, the diameter of inhibition zone of compound acidifier, plant extract combination and their combination on Escherichia coli and Staphylococcus aureus was determined by Oxford cup method. The compound acidifier is compounded according to the ratio of 15 parts of formic acid, 20 parts of acetic acid, 25 parts of glyceryl butyrate, 15 parts of lactic acid, and 5 parts of ethylenediamine tetraacetic acid; 20 parts of cinnamaldehyde and 10 parts of flavonoids.
[0021] Take 10g of the compound acidulant, 10g of the plant extract and the combination of 10g of the compound acidulant and the plant extract into 100ml of physiological saline, and mix on a magnetic stirrer for 30 minutes. Take Escherichia coli and Staphylococcus aureus cultured slant respectively, wash down the colony with appropriate amount of normal saline, insert it into the sterilized LB medium according to 4%, mi...
Embodiment 2
[0028] The compound acidulant is 15 parts by weight of glyceryl formate, 20 parts of glyceryl acetate, 25 parts of glyceryl butyrate, 15 parts of glyceryl lactate, and 5 parts of ethylenediaminetetraacetic acid; 10 parts of thyme essential oil, 40 parts of boluohui alkaloids, 20 parts of cinnamaldehyde, and 10 parts of flavonoids.
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