Method for performing STR typing on forensic mixed DNA sample

Abstract

The invention discloses a method for performing STR typing on a forensic mixed DNA sample. The invention provides a method for performing STR typing on a biological sample; and the method comprises the following steps of: extracting genome DNA of the biological sample, performing droplet type digital PCR amplification by taking the genome DNA as a template, sequencing an amplification product, andperforming STR typing according to a sequencing result, wherein the biological sample is a mixed biological sample. The invention also protects a method for performing STR typing on a DNA sample; andthe method comprises the following steps of: performing droplet type digital PCR amplification by taking the DNA sample as a template, sequencing an amplification product, and performing STR typing according to a sequencing result, wherein the DNA sample is a mixed DNA sample. The method provided by the invention has a huge application prospect in forensic DNA inspection.

Description

technical field [0001] The invention relates to a method for performing STR typing on forensic mixed DNA samples. Background technique [0002] With the improvement of the level of DNA testing, micro-contact test materials have gradually become the main test materials in DNA laboratories, and most of the samples obtained from such test materials are mixed DNA typing (such as the DNA of victims and suspects). admixture, admixture of two or more suspect samples, or admixture of a suspect with another unrelated individual, etc.). It is precisely these mixed samples that are of great practical significance in providing case clues, establishing the scope of investigation and providing court evidence. However, the DNA profile of the mixed sample is different due to the number of people mixed and the mixing ratio, resulting in many STR peak spectra, complex band patterns, and unstable peak patterns, resulting in difficult analysis of mixed sample results and high qualitative risks...

AI Technical Summary

Problems solved by technology

Among them, single cell separation technology depends on professional micromanipulation equipment and well-trained operators, and the conditions for single cell separation are limited

However, based on computer technology or manual splitting based on experienced experts, both rely on the acquisition of high-quality mixed maps, and cannot change the initial state of DNA mixing in the sample.

However, in actual cases, the possible mixture of the test material itself is that the victim’s DNA background is strong, while the suspect’s DNA content is relatively low, and the low-content DNA template is submerged in the ocean of high-content DNA templates.

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