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Identification method for alkali-resistant kiwi fruit germplasm resources

An identification method, kiwifruit technology, applied in horticultural methods, botanical equipment and methods, instruments, etc., can solve problems such as malnutrition, limited land area, and impact on market competitiveness, so as to improve accuracy and reliability, and ensure stability Consistency and consistency, the effect of identifying the structure accurately and reliably

Pending Publication Date: 2020-09-29
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, rootstocks are randomly selected in the production of kiwifruit in my country, resulting in uneven fruit appearance and internal quality of scion varieties, which affects market competitiveness.
Because kiwifruit likes acidic soil, the root system is shallow and fleshy, so the land area suitable for kiwifruit planting in my country is limited. The soil in most parts of the north is alkaline, and the pH value is generally around 8.0-8.5, which is very unfavorable for the growth of kiwifruit. Alkaline soil can cause yellowing of kiwifruit leaves, malnutrition and tree vigor
There are few domestic researches on the screening and identification of alkali-tolerant kiwifruit germplasm resources, and there is a lack of systematic and reliable identification standards and methods

Method used

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  • Identification method for alkali-resistant kiwi fruit germplasm resources
  • Identification method for alkali-resistant kiwi fruit germplasm resources
  • Identification method for alkali-resistant kiwi fruit germplasm resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] In this embodiment, 8 kinds of kiwifruit germplasm resources collected are taken as an example, numbered respectively as a, b, c, d, e, f, g, and h.

[0061] The dormant branches of 8 kinds of kiwifruit germplasm resources grafted in the resource nursery in the previous year were selected, and the branches of appropriate length were cut as materials for tissue culture. According to the distribution of bud points, cut the clipped branches into 40cm branch segments, and ensure that each branch segment has 2 bud points. Then use a small brush to gently brush off the dust and debris on the branch sections, being careful not to touch the young shoots. Then soak the branch segment in water added with 0.5% Tween-20 for 10 minutes, take it out and wash it with distilled water for 5 times, and then soak it in water. When soaking, immerse 1 / 3 of the branch section in the water and keep the water depth. When the water level drops, add water in time. After soaking for 8 weeks, n...

Embodiment 2

[0066] The alkali damage simulation treatment result in embodiment 1 and comparative example 1 is carried out the statistical analysis of alkali damage index:

[0067] Among them, the grading standard of alkali damage index is as follows:

[0068] Ⅰ The number of roots is 4-5, the root length is 3-4cm, and the root diameter is less than 1.5mm;

[0069] Ⅱ The number of roots is 2-3, the root length is 1-2cm, and the root diameter is 1.6-2.0mm;

[0070] Ⅲ The number of roots is 1, the root length is 0.5-1cm, and the root diameter is 2.1-2.5mm;

[0071] ⅣNo roots, growth is inhibited, and plants are dwarfed.

[0072] The calculation formula of alkali damage index is: ADI=∑(x×n) / (4N)×100

[0073] x--alkali damage series at all levels;

[0074] n--the number of plants corresponding to the alkali damage series at all levels;

[0075] N—the total number of plants investigated.

[0076] The basis for the identification of alkali resistance is the ADI value: the larger the ADI va...

experiment example 1

[0096] The statistical analysis result of the alkali damage index of experimental example 1 is as follows:

[0097] Table 3 Statistical Analysis Results of Alkali Damage

[0098]

[0099] From the identification results in Table 3, it can be seen that a, b, e, f are alkali-tolerant germplasm resources, d, h are moderate alkali-tolerant germplasm resources, and c, g are non-alkali-tolerant germplasm resources.

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Abstract

The invention belongs to the technical field of agricultural fruit tree germplasm resource identification, and provides an identification method for alkali-resistant kiwi fruit germplasm resources. The identification method for the alkali-resistant kiwi fruit germplasm resources comprises the following steps: (1) culturing of kiwi fruit tissue culture seedlings; (2) alkali damage simulation treatment of the tissue culture seedlings; (3) statistical analysis of alkali damage indexes; and (4) alkali resistance identification. The identification method provided by the invention improves the accuracy and reliability of identification results.

Description

technical field [0001] The invention belongs to the technical field of identification of agricultural fruit tree germplasm resources, and in particular relates to an identification method of basic kiwifruit germplasm resources. Background technique [0002] Kiwi fruit is an important fruit tree resource native to my country, and is loved by people because it is rich in vitamin C, folic acid and dietary fiber. The average annual output of kiwifruit in my country accounts for more than half of the export share in the world market, among which the output of kiwifruit in four provinces of Sichuan, Shaanxi, Henan and Guizhou accounts for more than 90% of the domestic production, but the overall quality, unit output and price of the fruit are comparable to New Zealand, Compared with developed countries such as Chile, there is still a big gap. One of the reasons is that my country lacks special rootstocks with strong adaptability and high resistance. At present, rootstocks are rand...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/06G16B40/00
CPCA01G7/06A01H4/008G16B40/00
Inventor 钟云鹏方金豹齐秀娟陈锦永孙雷明顾红王然林苗苗
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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