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Application of GSK-3beta as a blood marker in preparation of mental disorder early-diagnosis reagent

A technology for blood markers and mental disorders, applied in the field of early diagnostic reagents for autism spectrum disorders, can solve problems such as unclear expression of related proteins, and achieve the effect of easy discovery

Active Publication Date: 2020-09-22
NANTONG UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Most of the studies on markers related to mental disorders reported in the prior art focus on brain tissue. Due to the existence of the blood-brain barrier and tissue differences, the expression of these related proteins in the blood is not clear

Method used

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  • Application of GSK-3beta as a blood marker in preparation of mental disorder early-diagnosis reagent
  • Application of GSK-3beta as a blood marker in preparation of mental disorder early-diagnosis reagent
  • Application of GSK-3beta as a blood marker in preparation of mental disorder early-diagnosis reagent

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Embodiment 1

[0021] ASD model mice were constructed according to literature reports (Lieberman, O.J., V. Cartocci, et al. (2020). "mTOR Suppresses Macroautophagy During Striatal Postnatal Development and IsHyperactive in Mouse Models of Autism Spectrum Disorders." Front Cell Neurosci14:70.) . Construction method: Two-month-old C57 / BL6J mice were mated with a male-to-female ratio of 1:1. Female mice were intraperitoneally injected with 600mg / kg VPA on the 12th day of pregnancy.

[0022] 200 μl of blood was collected from newborn mice, and 5 times the volume of lysate (50mM Tris-HCl, pH7.4, 8.5% Sucrose, 10mM β-ME, 2mM EDTA, 50mM NaF, 1mM Na 3 VO 4 And protease inhibitor aprotinin, pepstatin, leupeptin each 10μg / ml, 1.0mM 4-(2-aminoethyl)benzenesulfonyl fluoride hy-drochloride (AEBSF)), ground and cracked in an ice-water bath. After lysis, add an equal volume of 2×Laemmli SDS samplebuffer, and cook in a boiling water bath for 5 minutes. Cooled samples were cooled using Pierce TM 660nm P...

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Abstract

The invention discloses an application of GSK-3beta as a blood marker in the preparation of a diagnostic reagent for mental disorders, especially autism spectrum disorders (ASD). GSK-3beta can be usedas an ASD early-diagnosis reagent, and a detection time window is 0-3 months for newborns. The non-invasive auxiliary diagnosis of ASD can be carried out by only using a small amount of blood sample,the large-scale infant ASD screening is facilitated, and early discovery, early intervention and early treatment of ASD are facilitated.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of GSK-3β as a blood marker in the preparation of early diagnosis reagents for mental disorders, especially autism spectrum disorders. Background technique [0002] Glycogen synthase kinase 3 (GSK-3) is a serine / threonine protein kinase that regulates the metabolism, growth and development, apoptosis and other processes of the body through multiple signaling pathways such as Wnt / -catenin, and is essential for the survival of the body. essential substance. A large number of studies have shown that abnormal regulation of GSK-3 can activate specific cellular pathways and circuits, thereby inducing mental disorders, such as bipolar disorder, depression, autism, and schizophrenia. Mammalian GSK-3 has two subtypes: GSK-3α and GSK-3β, which are expressed at high levels in brain tissue. GSK-3α is mainly expressed in the Purkinje cells of the cerebral cortex, hippo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/48G01N33/68
CPCC12Q1/6883C12Q1/485G01N33/6893C12Q2600/158G01N2800/30Y02A50/30
Inventor 储丹丹顾锦华伍倩沈鑫
Owner NANTONG UNIVERSITY
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