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Application of IL-8 positive initial T cell as target for diagnosing thymus space-occupying diseases

A technology of IL-8, 1.IL-8, applied in the field of disease diagnosis or diagnostic reagents

Pending Publication Date: 2020-09-22
INST PASTEUR OF SHANGHAI CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of IL-8 positive initial T cell as target for diagnosing thymus space-occupying diseases
  • Application of IL-8 positive initial T cell as target for diagnosing thymus space-occupying diseases
  • Application of IL-8 positive initial T cell as target for diagnosing thymus space-occupying diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, sample processing and detection method

[0063] 1. Sample processing

[0064]Peripheral blood mononuclear cells were isolated from patients' preoperative and follow-up samples by density gradient centrifugation. The specific operation is as follows: add 5ml Ficoll to the 15ml EP tube. Take 5ml of anticoagulated blood sample and mix it with PBS at a ratio of 1:1. Then carefully superimpose the mixed blood sample on the Ficoll, pay attention to keep the interface between Ficoll and blood sample clear. The sample was then placed in a centrifuge and centrifuged at 500 g for 20 minutes at room temperature, with the centrifuge speed adjusted to a minimum. After centrifugation, aspirate as much as possible the yellow transparent layer of the upper PBS and plasma mixture and discard it, then carefully aspirate the buffy coat layer rich in peripheral blood mononuclear cells. Transfer the buffy coat to another 15ml EP tube, add 3 times the volume of medium, and ...

Embodiment 2

[0072] Example 2, patients with thymomatosis and other thymus occupying diseases are identified by CT and IL8 in blood + Comparison of Ratio Identification of Naive T Cells

[0073] Patients with thymic cysts and thymoma were selected, and traditional imaging methods were used for diagnosis. The images of two representative patients are as follows figure 1 As shown in A. It can be seen from the figure that the two space-occupying lesions are very similar in imaging, and it is difficult to make an accurate identification based on the images alone.

[0074] Next, the peripheral blood of the two patients was obtained, and analyzed by flow cytometry as described in Example 1. figure 1 B is the flow cytometry diagram of IL8 in the corresponding naive T cells. Among them, naive T cells refer to CD15 - CD235 - CD3 + CD8 - CD45RA + CCR7 + cells (CD4 + Naive T cells; since CD4+T cells are stimulated by PMA+Ionomycin, the expression level of CD4 is down-regulated, and it is di...

Embodiment 3

[0077] Example 3. Analysis of the proportion of IL8-positive naive T cells in patients with different thymic space-occupying diseases

[0078] The inventors collected peripheral blood mononuclear cells from 35 thymoma patients, 35 thymic cyst patients, 10 thymic squamous cell carcinoma patients, 5 thymic hyperplasia patients, 8 germ cell tumors and 7 lymphoma patients. Using the method as in Example 1 above, flow cytometry was used to detect the presence of IL-8 positive cells.

[0079] The measurement results and the ratio of IL8-positive initial T cells in patients with different thymic space-occupying diseases are shown in Tables 1-2.

[0080] Table 1, IL-8 + CD4 naive T cell ratio

[0081]

[0082] Table 2, IL-8 + CD8 naive T cell ratio

[0083]

[0084] The above results show that, when the levels of IL-8-positive naive T cells before stimulation with phorbol ester (PMA) and ionomycin (Ionomycin) are similar, the levels of IL-8 naive T cells in thymoma and thymu...

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Abstract

The invention relates to application of an IL-8 positive initial T cell as a target for diagnosing thymus space-occupying diseases. The invention reveals that the IL-8 positive initial T cell in peripheral blood has significant meaning for diagnosis or typing of thymus space-occupying diseases, and can assist in diagnosis of thoracic adenoma by analyzing the proportion and function of the IL-8 positive initial T cell migrated from thymus in peripheral blood. According to the technical scheme, the accuracy of diagnosis or typing of thymus space-occupying diseases can be remarkably improved.

Description

technical field [0001] The invention belongs to the field of disease diagnosis or diagnostic reagents, and more specifically, the invention relates to the application of IL-8 positive T cells in the diagnosis of thymus occupying diseases. Background technique [0002] Thymus occupying diseases include thymic hyperplasia, thymic cyst, thymoma, thymic squamous cell carcinoma, lymphoma, germ cell tumor and metastatic tumor. Thymoma, the most common mediastinal tumor in humans, is a tumor of thymic epithelial cells (TEC). Thymoma can occur at almost any age (thymoma cases from 8 months to 90 years old have been reported), with various histological manifestations, unpredictable disease development and risk of malignant transformation, ranging from asymptomatic to a series of syndromes with neoplasia, often Accompanied by immune system abnormalities and autoimmune diseases, myasthenia gravis is the most common. Therefore, accurate diagnosis of thymoma is crucial. [0003] At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886C12Q1/6888C12Q1/04G01N33/68G01N33/574G01N33/569
CPCC12Q1/6883C12Q1/6886C12Q1/6888G01N33/6893G01N33/57407G01N33/57484G01N33/57492G01N33/56966
Inventor 王海坤丁建勇高士麟蒋家好金淳李俊翔
Owner INST PASTEUR OF SHANGHAI CHINESE ACADEMY OF SCI
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