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Germination growth-promoting bacteria composition and application thereof

A microbial composition and microbial technology, applied in the field of microorganisms, can solve the problems of irrigation water loss, water shortage, short stay around seeds or roots, etc., and achieve the effect of improving soil quality, increasing seed germination rate, and improving plant growth

Active Publication Date: 2020-09-18
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in non-rainy season Tibetan agricultural planting areas, lack of water, more than 80% of irrigation water is lost in the form of evaporation and infiltration, and only about 10% of water can stay around plant seeds or roots for a short time, and insufficient water management often becomes a limitation The main reason for its germination

Method used

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  • Germination growth-promoting bacteria composition and application thereof
  • Germination growth-promoting bacteria composition and application thereof
  • Germination growth-promoting bacteria composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Screening of each bacterial strain in the germination-promoting bacteria composition

[0038] 1. Prepare culture medium.

[0039] (1) LB liquid medium: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH=7.0, sterilized at 121°C for 20min. Add 18 g of agar to LB liquid medium to obtain LB solid medium.

[0040] (2) Germination hormone fermentation medium: bean cake powder 10g, fish meal 10g, corn flour 5g, yeast extract 5g, C 6 h 5 o 7 (NH 4 ) 3 0.5g, MgSO 4 ·7H 2 O 0.25g, FeSO 4 ·7H 2 O 0.25g, CaCO 3 3.0g, NaCl 2.5g, CoCl 2 0.1g, K 2 HPO 4 0.3g, NaHCO 3 0.15g, distilled water 1000mL, pH=7.5.

[0041] (3) Gaoshi No. 1 liquid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, pH=7.4~7.6. After adding 12 g of agar in Gao Shi No. 1 liquid medium, Gao Shi No. 1 solid medium was obtained.

[0042] (4) PDA liquid medium: Weigh 200g of peeled potatoes, ...

Embodiment 2

[0059] Embodiment two: the influence of germination active growth-promoting bacteria on the germination of highland barley seeds

[0060] Seed soaking in bacterial solution was used to verify whether each microorganism obtained in Example 1 had the effect of promoting germination of highland barley seeds, accelerating nutrient transformation of endosperm, increasing the number of roots, and improving the stress resistance of seedlings.

[0061] The 3 bacterial strains (Bacillus subtilis YX7, Pseudomonas KSX1-2 and Bacillus megaterium CGMCC 1.1870) obtained by embodiment one screening were scraped an inoculating loop respectively, and were inoculated in the medium containing 300mL sterilized beef extract peptone liquid medium. In a 500mL Erlenmeyer flask, cultivate on a shaking table at 30°C and 180r / min until the cell concentration is about 10 9 Stop at CFU / mL to obtain each fermentation broth. Then take each fermented liquid and put it in a 250mL beaker respectively to obtai...

Embodiment 3

[0074] Embodiment three: the screening of other materials except microorganisms in the seed coating agent

[0075] 1. Screening of film-forming materials.

[0076] Candidate materials include: hydroxyethyl cellulose (HC), sodium carboxymethyl cellulose (CMC), gum arabic (GA), polyvinyl alcohol (PVA).

[0077] Dissolve each candidate material with hot water (70-80° C.) under the agitation of a magnetic stirrer, and prepare 5% hydroxyethyl cellulose solution and 5% sodium carboxymethyl cellulose solution according to mass percentage, respectively. 5% gum arabic solution, 5% polyvinyl alcohol; and enter the test as follows:

[0078] Film-forming time test: Take an appropriate amount of each prepared film-forming agent on a clean slide, observe the film-casting process and time it with a stopwatch, so as to understand the film-forming time of different film-forming agents.

[0079] Film-forming test: Place the prepared film-forming agent solutions on a clean glass slide (10×2cm)...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a germination growth-promoting bacteria composition and an application thereof. According to the specific technical scheme, the composition comprises bacillus subtilis, pseudomonas and bacillus megatherium; 16S rDNA of the bacillus subtilis is as shown in SEQ ID NO:1, and 16S rDNA of the pseudomonas is as shown in SEQ ID NO:2; and a collection number of the bacillus megatherium is CGMCC 1.1870. The invention provides a novel microbial composition; the microbial composition can independently serve as a seed soaking agent or a component of a seed coating agent to be applied to promotion of seed germination; and the microbial composition can also be used as a component of bio-organic fertilizer to helpimprove the soil quality, increase the seed germination rate and improve the plant growth condition.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a germination-promoting bacteria composition and its application. Background technique [0002] Highland barley has a short growth period and is rich in nutrients, and the straw can be used as forage for livestock in winter, and is widely planted in plateau agricultural areas. However, strong solar radiation, large water evaporation, and obvious precipitation variability in Tibetan areas, the high rainfall and the misalignment of the peak water consumption period of crops, coupled with shallow farming soil and weak soil water storage capacity, lead to easy loss of soil moisture and frequent droughts of crops . The statistical results show that the rainfall in the Tibetan area during the highland barley planting season (early April) is about 1.90-14.7mm, and the area of ​​sprouting setbacks caused by drought can reach 30%-50% of the total highland barley planti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/20C05G3/80A01N63/22A01N63/27A01P21/00C09K17/14C09K17/32C12R1/125C12R1/38C12R1/11C09K101/00
CPCC12N1/20C05D1/00C05G3/00C05G3/20C05G3/80A01N63/22A01N63/27C09K17/14C09K17/32C09K2101/00C05F11/00C05F11/02C05F11/08
Inventor 李东陈意超
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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