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Flos chinensis extract, its preparation method and use

A technology of dense Mongolian flowers and extracts, applied in the field of medicine, can solve problems such as unknown, unresearched, complex chemical components, etc., and achieve the effects of improving retinal degenerative diseases, inhibiting cell death, and preventing and treating thickness.

Active Publication Date: 2021-09-17
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prescription is composed of six medicinal materials including Astragalus, Ligustrum lucidum, Motherwort, Coptis chinensis, Cinnamon and Mimenghua. Pharmacological activity of HUVEC proliferation, which pharmacological components can act on VEGF-VEGFR signaling pathway and have pharmacological activity of inhibiting HUVEC proliferation under hypoxia cannot be known from its disclosure

Method used

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  • Flos chinensis extract, its preparation method and use
  • Flos chinensis extract, its preparation method and use
  • Flos chinensis extract, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0094] Preparation Example 1: Water extraction and alcohol extraction to extract the active ingredients in the flower

[0095] Weigh 100 g of Chinese herbal medicine pieces of M. japonica, add 1000 ml of 95% ethanol, infiltrate for 0.5 h, heat under reflux at 80 °C for 1 h, and collect the filtrate by filtration; add 1000 ml of 95% ethanol to the filter residue again, extract under reflux at 80 °C for 1 h, filter and combine twice The filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a 95% concentrated solution of the floristry florets extract, which was freeze-dried (19.6 g), number MMH-95.

[0096] After 95% ethanol extraction, 1000 ml of 50% ethanol was added to the filter residue, heated and refluxed at 100 °C for 1 h, and the filtrate was collected by filtration; 1000 ml of 50% ethanol was added to the filter residue again, heated and refluxed at 100 °C for 1 h, filtered and combined two filtrates, and the filtrate was reduced by rotary e...

preparation Embodiment 2

[0100] Preparation Example 2: Water Extraction-Solvent Extraction Method Separation of Active Components in Bulgari

[0101] Weigh 100 g of Chinese herbal medicine pieces of Mimonella japonica, add 1000 ml of distilled water, infiltrate for 0.5 h and then heat and reflux at 100 °C for 1 h, filter and collect the filtrate; add 1000 ml of distilled water to the filter residue again, heat and reflux for 1 h at 100 °C, filter and combine the filtrates twice, and use rotary Evaporator was concentrated under reduced pressure to obtain 200ml of Mimeng flower water extract concentrate, 200ml of ethyl acetate solvent was added for extraction, and after standing to separate layers, the upper layer of ethyl acetate solution was collected. Press-dried to obtain ethyl acetate extraction sample, number MMH-H 2 O-EA; add 200ml of n-butanol solvent to the remaining water layer for extraction, let stand until the layers are separated, collect the upper n-butanol solution, extract 5 times by th...

preparation Embodiment 3

[0102] Preparation Example 3: Purification and Identification of Compounds I to V

[0103] Weigh the MMH-C-H obtained in Preparation Example 2 2 5 g of sample O was dissolved by adding an appropriate amount of distilled water to heat and ultrasonically dissolved, and macroporous resin (D101) was used for column chromatography separation (500 × 60 mm), firstly washed with 1000 ml of distilled water, and then washed with 1000 ml of 20% ethanol and collected the eluate. The deliquoring was analyzed by HPLC to contain main characteristic peaks, and the purity was above 70%. The samples were concentrated and combined to obtain 20% ethanol elution samples. The sample was purified again with ODS chromatography packing, eluted with distilled water and 10% methanol in sequence, concentrated and combined with 10% methanol eluent, and analyzed by HPLC, the purity was 94.14%. Concentrate and dry under reduced pressure to obtain 86.2 mg of brown-yellow solid powder, No. Compound I, which ...

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Abstract

The invention discloses an extract of Budisma japonica, its preparation method and its application in the preparation of medicines for treating or preventing retinal degeneration. The present application adopts aqueous solution to extract Budisma japonica, and obtains a kind of Budisma japonica extract, in which apigenin-7,4'-di-O-β-D-glucuronide, echinacoside The total weight of the five compounds of , verbascoside, isovabacoside and apigenin-7-O-rutinoside accounts for more than 10% of the total weight of the extract. It has been verified by experiments that the above extracts have good inhibitory activity on both VEGFR-1 and VEGFR-2, and can significantly inhibit the occurrence of degeneration of retinal photoreceptor cells, and can be used to treat or prevent retinal degeneration.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular, the present invention relates to an extract of Flos chinensis, its preparation method, and its use in preparing a medicine for treating or preventing retinal degenerative diseases, and the extract containing the Flos chinensis pharmaceutical composition of the substance. Background technique [0002] Retinal degenerative disease is a type of fundus disease with retinal photoreceptor cell death as the core pathological change, which can cause severe visual impairment and even blindness. (age-related macular degeneration, AMD), diabetic retinopathy (diabetic retinopathy, DR) and retinitis pigmentosa (retinitis pigmentosa, RP) and other diseases. Among them, AMD is the leading cause of low vision and blindness in the elderly in developed countries. There are about 30 million patients with age-related macular degeneration in the world, and about 500,000 people are blinded by it every...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H15/18C07H1/08A61K31/7048A61K31/7032A61P27/02
CPCA61P27/02C07H1/08C07H15/18C07H17/07
Inventor 张勇陈瑜李波杜霄烨徐静徐志建蔡婷婷张腾朱维良
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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