A kind of breeding method of natural gynogenesis dory

Abstract

The invention discloses a method for cultivating natural gynogenetic bream. The bream is used as the female parent, and the yellow-tailed pomfret is used as the male parent. To induce labor, the dose of LRH‑A is 8‑10 μg / kg, and the dose of HCG is 400‑500 IU / kg; inject the female parent fish first, and then artificially inject the mixed oxytocic agent of LRH‑A and HCG to the father parent fish after 3 hours. To induce labor, the injection dose is half of that of the parent fish, and the intraperitoneal injection method is adopted at the base of the pectoral fin without scales; after artificial insemination, running water incubation, breeding, testing and screening, the natural gynogenetic bream is obtained. The cultivation method of the present invention changes the activity of the eggs and sperm of the parents, and then obtains the natural gynogenetic bream that is sexually fertile. During the entire cultivation process, technical treatments such as inactivation of sperm and doubling of eggs are not required, which greatly improves Reduced workload.

Description

technical field [0001] The invention relates to a method for distant hybrid breeding of fish, in particular to a method for preparing natural gynogenetic group bream by distant hybridization between the bream and the subfamilies of the subfamily Yellow-tailed pomfret. Background technique [0002] Distant hybridization refers to the hybridization between species whose genetic relationship is interspecific or above, which can transfer the genome from one species to another, resulting in changes in the genotype and phenotype of the offspring. Distant hybridization can also produce gynogenetic offspring, so that natural gynogenetic offspring show heterozygous effects in terms of meat quality, disease resistance, stress resistance, and growth speed, and thus form sexually fertile offspring through distant hybridization. Natural gynogenetic offspring provide an important germplasm resource bank for genetic breeding. How to use the existing technology to form a new type of natura...

AI Technical Summary

Problems solved by technology

However, since bream is a fish sensitive to low oxygen, low oxygen content causes huge economic losses during the breeding process. In addition, bream grows relatively slowly

In the existing technology, the operation process of obtaining gynogenetic bream is cumbersome, and the selection requirements for sperm parent, sperm inactivation method, and chromosome doubling method are very strict, and the obtained gynogenetic bream are all female offspring , these deficiencies limit the application of gynogenetic bream in production practice

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