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Antibody array card for detecting three mycotoxins by using difunctional antigen-guided universal signal

A mycotoxin and dual-function technology, applied in the field of food and drug safety testing, can solve the problems of orderly release of immune chip signal probes, complex specific recognition and background control, misjudgment, probe mutual interference, etc., and achieve active protection Favorable, improve detection sensitivity and quantitative precision, improve detection signal strength and sensitivity

Pending Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The orderly release, specific recognition and background control of immune chip signal probes are relatively complex issues, and mutual interference between probes is prone to cause misjudgment of results

Method used

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  • Antibody array card for detecting three mycotoxins by using difunctional antigen-guided universal signal
  • Antibody array card for detecting three mycotoxins by using difunctional antigen-guided universal signal
  • Antibody array card for detecting three mycotoxins by using difunctional antigen-guided universal signal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] An antibody array card for the detection of three mycotoxins with a dual-function antigen-guided universal signal, including a liner 5, and a dual-function antigen release pad 1 that is sequentially adhered to the liner 5 and partially overlapped adjacent to each other, and a universal fluorescent light Probe release pad 2, cellulose acetate membrane 3, and water-absorbent pad 4; the bifunctional antigen release pad contains the detection antigen formed by coupling ovalbumin with FB1, DON, and F-2 respectively. 7-amino-4-hydroxyl-2-naphthalenesulfonic acid" label 62; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with anti-7-amino-4-hydroxyl-2-naphthalenesulfonic acid antibody 65 Probe, the fluorescent probe is a quantum dot 61; the cellulose acetate membrane 3 is provided with detection line A, detection line B, detection line C and quality control line 34, the detection line A, detection line B, detection line Line C immobi...

Embodiment 2

[0056] For the detection method of simultaneously detecting FB1, DON and F-2, refer to Figure 6 ,Proceed as follows:

[0057] Take 5g of crushed cereal crop samples, fully extract the sample in 25mL of 70% methanol-water solution, centrifuge at 5000rpm for 10 minutes, take 2.0mL of the supernatant and add it to 8.0mL of PBS buffer, shake well to become the sample solution; use a dropper Add 3-4 drops of sample solution on the bifunctional antigen release pad. During the movement of the sample solution to the nitrocellulose membrane, the bifunctional antigen and the universal fluorescent probe are released, and successively cross the detection line and the quality control line. According to the detection line A, detection line B, detection line C and the fluorescent signal on the quality control line to determine whether the sample contains FB1, DON and F-2.

[0058] refer to Figure 5 . The FB1 antibody 311 immobilized on the nitrocellulose membrane detection line A, and t...

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Abstract

The invention relates to an antibody array card for detecting three mycotoxins by using a difunctional antigen-guided universal signal. The antibody array card comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by respectively coupling ovalbumin with FB1, DON and F-2; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with detection lines A, Band C and a quality control line, the detection lines A, B and C are respectively used for fixing an anti-FB1 antibody, an anti-DON antibody and an anti-F-2 antibody, and the quality control line isused for fixing the anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, andthe quality control reliability is improved; the signal intensity and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to an antibody array card for detecting three mycotoxins with a dual-functional antigen-guided universal signal. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. At present, more than 300 mycotoxins with different chemical structures are known, among which there are more than 30 mycotoxins produced by fungal strains, which are carcinogenic, genotoxic, and teratogenic to humans and animals. It can cause hepatotoxicity, nephrotoxicity, reproductive abnormalities and suppress immune response, which is extremely toxic and harmful. [0003] The possibility that agricultural products may be contaminated by a variety of fungi, which metabolize to produce mixed contamination of a variety of mycotoxins, is attracting worldwide attention. Authoritative studies at home and abroad have shown that peanuts, corn, soybeans and other grain and oil ...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558G01N33/53
CPCG01N33/582G01N33/588G01N33/558G01N33/5308
Inventor 郭杰标
Owner SHAOGUAN COLLEGE
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