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Sculellaria barbata glycan, extraction method thereof, and application of sculellaria barbata glycan in preparation of anti-SARS-CoV-2 drugs

An extraction method, the technology of Scutellaria barbata, applied in the field of medicine

Active Publication Date: 2020-08-21
爱礼康生物医药(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the anti-SARS-CoV-2 of Scutellaria barbatae

Method used

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  • Sculellaria barbata glycan, extraction method thereof, and application of sculellaria barbata glycan in preparation of anti-SARS-CoV-2 drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the extracting method of Scutellaria barbata polysaccharide comprises the following steps:

[0025] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0026] Step 2. Add 14 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.8 to obtain a mixed solution, heat the mixed solution to 55°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 40min, obtain enzymolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.6%, 0.6%, 0.5% of Scutellaria barbata powder mass;

[0027] Step 3: Heating the enzymolysis solution to 105°C and maintaining it for 1 hour, then cooling and filtering to obtain the filtrate;

[0028] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with running water for 2 days to obtain a dialysate;

[0029] Step 5, heat and concentrate the dia...

Embodiment 2

[0031] Embodiment 2: the extracting method of Scutellaria barbata polysaccharide comprises the following steps:

[0032] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0033] Step 2. Add 12 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.6 to obtain a mixed solution, heat the mixed solution to 60°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 45min, obtain enzymatic hydrolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.5%, 1%, 0.7% of Scutellaria barbata powder mass;

[0034] Step 3, heating the enzymolysis solution to 110°C and keeping it for 1 hour, then cooling and filtering to obtain the filtrate;

[0035] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with running water for 3 days to obtain a dialysate;

[0036] Step 5, heat and concentrate the ...

Embodiment 3

[0038] Embodiment 3: the extracting method of Scutellaria barbata polysaccharide, comprises the following steps:

[0039] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0040] Step 2. Add 15 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.7 to obtain a mixed solution, heat the mixed solution to 50°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 35min, obtain enzymolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.8%, 0.5%, 0.4% of Scutellaria barbata powder mass;

[0041] Step 3, heating the enzymolysis solution to 100°C and keeping it for 1.2h, then cooling and filtering to obtain the filtrate;

[0042] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with running water for 3 days to obtain a dialysate;

[0043] Step 5, heat and concentrate the dialysat...

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Abstract

The invention discloses sculellaria barbata glycan, an extraction method thereof, and an application of the sculellaria barbata glycan in the preparation of anti-SARS-CoV-2 drugs, and belongs to the technical field of drugs. The method for preparing the sculellaria barbata glycan comprises the following steps: 1, crushing dried sculellaria barbata plants to obtain sculellaria barbata powder; 2, adding deionized water into the sculellaria barbata powder, adjusting the pH value of the sculellaria barbata powder to 4.6-4.8 to obtain a mixed solution, heating the mixed solution to 50-60 DEG C, then adding cellulase, pectinase and papain, and performing enzymolysis for 35-45 min to obtain an enzymolysis solution; 3, heating the enzymolysis solution to 100-110 DEG C, keeping the temperature for1-1.2 hours, and then cooling and filtering to obtain a filtrate; 4, heating and concentrating the filtrate to obtain a concentrated solution, and dialyzing the concentrated solution with flowing water for 2-3 days to obtain a dialysate; 5, heating, concentrating and centrifuging the dialysate, removing obtained precipitate, adding 95% ethanol with the volume 5-6 times that of the obtained supernatant, and standing overnight for out alcohol precipitation to obtain an alcohol precipitation solution; and 6, carrying out centrifugal treatment on the alcohol precipitation solution to obtain a precipitate, and alternately washing the precipitate with absolute ethyl alcohol and acetone for 3-5 times to obtain the sculellaria barbata glycan.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to barbata polysaccharides, an extraction method and an application in preparing anti-SARS-CoV-2 medicines. Background technique [0002] 2019 Novel Coronavirus, also known as SARS-CoV-2. Common signs of human infection with SARS-CoV-2 include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. Currently, there is no specific treatment for the disease caused by the novel coronavirus. [0003] The new coronavirus pneumonia virus SARS-Cov-2 is a typical coronavirus 2-5, and the diameter of the virus particle is about 60-220nm. There are three kinds of glycoproteins on the membrane surface: spike glycoprotein (S, Spike Protein), small envelope glycoprotein (E, Envelope Protein) and membrane glycoprotein (M, Membrane Protein). Among...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/14A61P11/00
CPCC08B37/0003C08B37/006A61K31/715A61P31/14A61P11/00
Inventor 丁侃陈霞孙成新姚晓东王秋雨张艳丽
Owner 爱礼康生物医药(苏州)有限公司
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