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A nano drug preparation and its application in the prevention and control of banana wilt

A technology of banana fusarium wilt and banana fusarium wilt bacteria, applied in the biological field, to achieve the effect of reducing the incidence rate and disease index

Active Publication Date: 2021-05-18
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effect of MSNs on banana wilt has not been reported yet.

Method used

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  • A nano drug preparation and its application in the prevention and control of banana wilt
  • A nano drug preparation and its application in the prevention and control of banana wilt
  • A nano drug preparation and its application in the prevention and control of banana wilt

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Experimental program
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Effect test

Embodiment 1

[0036] 1. Materials and methods

[0037] 1.1 Materials

[0038] The tested antagonistic bacterial strain 5x-2, the tested pathogenic fungi: Banana Fusarium wilt race 4, Brazilian banana seedlings and MSNs with a particle diameter of 20 nm and a mesopore diameter of 3.0 nm were all obtained by the Institute of South Subtropical Crops, Chinese Academy of Tropical Agricultural Sciences. Provided by the Banana Research Group. Medium: solid PDA medium, solid / liquid LB medium.

[0039] 1.2 Methods

[0040] 1.2.1 Antibacterial experiments of MSNs

[0041] MSNs were added to the PDA medium to make the final concentrations of 0, 10, 20, 40, 60, and 100 mg / L, respectively. After sonication for 1 h, sterilized at 121 °C for 20 min, and then sonicated for 10 min to pour the plate; instructions The bacterium is the No. 4 race Foc4 of Fusarium wilt of banana, and the diameter of the No. 4 race of Fusarium oxysporum is taken to inoculate the 5mm mycelial block in the center of the medium...

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Abstract

The invention discloses a nano-medicine preparation and its application in the prevention and control of banana wilt, which belongs to the field of biotechnology. The invention studies nano-mesoporous silica particles (MSNs) and ethanol extracts of 5x-2 bacterial fermentation broth , MSNs loaded with 5x‑2 bacterial fermentation broth ethanol extract and its supernatant after loading treatment on the bacteriostatic effect of banana wilt, and the control effect and physiological and biochemical effects of MSNs and MSNs loading treatment group on banana wilt after inoculation It was found that MSNs had an inhibitory rate of 13.25%-16.55% against Fusarium wilt race 4; MSNs and MSNs loading treatment could reduce its incidence and disease index, laying the foundation for the development of new nano-pesticides for the prevention and control of Fusarium wilt.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nano-medicine preparation and its application in preventing and controlling banana fusarium wilt. Background technique [0002] Banana fusarium wilt is a highly devastating and widespread soil-borne fungal disease of plants caused by the infection of Fusarium oxysporuin f.sp.cubense (Foc). In recent years, Fusarium oxysporum has been divided into four physiological races, and their pathogenicity is also different, and the most pathogenic race is No. 4 physiological race. At present, there is no thorough and effective method to control banana fusarium wilt, and the control measures are mainly chemical control, biological control and breeding of disease-resistant varieties. Chemical control mainly uses chemical pesticides such as fungicides to prevent and control them. Long-term use of fungicides will cause environmental pollution, damage to human health, and produce drug-resistant ...

Claims

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Application Information

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IPC IPC(8): A01N63/20A01N59/00A01P3/00A01G13/00A01G22/05
CPCA01G13/00A01N59/00A01G22/05A01N63/20
Inventor 谢江辉孙德权陆新华李普旺胡会刚
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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