Degrading bacterium agent for kitchen waste treatment and application thereof
A technology of food waste and degrading bacteria, which is applied in the direction of microbial treatment, bio-organic part treatment, bacteria, etc., can solve the problems of unsuitable food waste degradation, and achieve various types of molecular structures, excellent surface properties, and extremely low biological toxicity Effect
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[0043] The degrading bacterial agent of the present invention, its preparation method and application thereof comprise the following steps:
[0044] (1) Bacteria activation culture
[0045] First, respectively inoculate Bacillus subtilis, Bacillus lysine, and Bacillus licheniformis into beef extract peptone liquid culture medium for activation culture, place them in a constant temperature shaker at a temperature of 30-35°C and 180-220rpm for 1-2 Days, to logarithmic growth phase, to obtain activated liquid seed solution.
[0046] The formula of beef extract peptone liquid medium is as follows: beef extract 3g, peptone 10g, yeast extract 2g, sodium chloride 5g, ultrapure water 1000mL, pH7.4-7.6.
[0047] Then Trichoderma viride, Candida glabrata and Yarrowia lipolytica strains were respectively inoculated into potato glucose medium for activation culture, placed on a constant temperature shaker at a temperature of 20-25°C and 180-220rpm for 2 -4 days, to logarithmic growth ph...
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