Bioactive substance freeze-dried solid mask and preparation method thereof
A bioactive, freeze-drying technology, applied in the field of skin care products, can solve the problems of fast expiration of cytokines, short storage time at room temperature, and low actual effect, and achieve the effects of softening stratum corneum protein, reducing wrinkle growth, and relieving stress.
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[0040] Preparation example of adipose-derived mesenchymal stem cell lysate
[0041] Preparation example 1 of adipose-derived mesenchymal stem cell lysate: A, solution preparation: add serum substitute to DMEM / F12 basal medium to make a medium containing 4% serum substitute, labeled as solution C; Add 20 mL of 0.25% trypsin to the saline, mix well, and mark it as solution D.
[0043] b1. Add 10mL of DMEM / F12 basal medium to a 15mL centrifuge tube, preheat to 37°C, and let stand for later use;
[0044] b2. After taking out the cryopreservation tube containing human adipose tissue-derived seed mesenchymal stem cells from the liquid nitrogen tank, quickly put it into a 37°C water bath, place the cryopreservation tube upright with the tube cap up and above the water surface, Shake the cryovial quickly to melt the cryopreservation solution in the cryovial quickly;
[0045] b3. After thawing the cryopreservation solution (seed mesenchymal stem cells) of ...
preparation example 2
[0098] Preparation example 2 of adipose-derived mesenchymal stem cell lysate: The difference between this preparation example and the preparation example 1 of adipose-derived mesenchymal stem cell lysate is that step d18 is not included, and the cell lysate is directly vacuumed after obtaining the cell lysate at d17 Freeze-dry to obtain adipose-derived mesenchymal stem cell lysate.
preparation example 3
[0099] Preparation example 3 of adipose-derived mesenchymal stem cell lysate: the difference between this preparation example and the preparation example 1 of adipose-derived mesenchymal stem cell lysate is that in step d18, β-cyclodextrin and trehalose are not added to the cell lysate.
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