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Screening method and application of SNP molecular markers associated with egg shell thickness, eggshell strength and egg shape index of Nandanyao chickens

A technology of molecular markers and screening methods, applied in the field of molecular biology, can solve the problems of genotype selection of target genes, low selection efficiency and accuracy, etc.

Active Publication Date: 2020-08-11
GUANGXI UNIV
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AI Technical Summary

Problems solved by technology

However, traditional breeding mainly relies on breeding experience to select offspring based on phenotype, and cannot directly select the genotype of the target gene
Therefore, the selection efficiency and accuracy are low, quantitative traits are controlled by multiple genes, and often one or several SNPs play a role in the gene

Method used

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  • Screening method and application of SNP molecular markers associated with egg shell thickness, eggshell strength and egg shape index of Nandanyao chickens
  • Screening method and application of SNP molecular markers associated with egg shell thickness, eggshell strength and egg shape index of Nandanyao chickens
  • Screening method and application of SNP molecular markers associated with egg shell thickness, eggshell strength and egg shape index of Nandanyao chickens

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Embodiment 1

[0034] The screening method of Nandanyao eggshell thickness, eggshell strength and egg shape index associated with SNP molecular markers, the specific steps are as follows:

[0035] (1) Consult the literature, query egg quality related genes, the related genes are ALAS1, CPOX-P6, TF, TSPO, SLC25A5 and SLLCO1A2 genes, design primers according to the gene sequence, the sequence of the primers is as shown in SEQ ID NO:12~ Shown in SEQID NO: 23; select 100 Nandan Yao chickens, collect blood to extract genomic DNA, mix the pool and send it to the company for sequencing, and detect the genes with mutant SNP sites on Nandan Yao chickens;

[0036] (2) Select 256 Nandanyao chicken hens provided by Guangxi Guigang Gangfeng Agriculture and Animal Husbandry Co., Ltd. as test chickens, and carry out single-cage breeding according to the company's breeding standards;

[0037] From the age of 24 weeks, the eggshell thickness, eggshell strength and egg shape index shape indicators of eggs wer...

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Abstract

The invention discloses a screening method of SNP molecular markers associated with egg shell thickness, eggshell strength and egg shape index of Nandanyao chickens. The method comprises the followingsteps: (1) taking Nandanyao chickens, sampling blood to extract genome DNA, performing sequencing, and detecting genes with mutation SNP sites on the Nandanyao chickens; (2) taking Nandanyao hens astest chickens, and measuring eggshell thickness, eggshell strength and egg shape index of eggs; (3) extracting genome DNA, carrying out multiple PCR sequencing on the SNP sites detected in the step (1), and collecting genotype information of the test chickens at the SNP sites; and (4) sorting sequencing data and eggshell thickness, eggshell strength and egg shape index data, and screening out candidate SNP molecular markers which are extremely and significantly associated with eggshell thickness, eggshell strength and egg shape index, wherein the Pr>F value is less than 0.01. The operation steps are simplified, the method is suitable for breeding of high-heredity quantitative characters, the breakage resistance of eggs is improved after screening, and transportation is convenient.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a screening method and application of Nandanyao eggshell thickness, eggshell strength and egg shape index-related SNP molecular markers. Background technique [0002] my country is a big chicken raising country, and its egg production ranks first in the world. However, defective eggs produced by egg shell quality problems have caused huge losses to enterprises. External egg quality and eggshell properties of eggs include eggshell thickness, eggshell strength, and egg shape index. Eggshell thickness and eggshell strength are important factors that affect egg transportation, preservation, incubation, and price. Eggshell strength is the main index of eggshell resistance to breakage, which is of great significance for packaging and transportation, and is also an important factor affecting the pass rate of breeding eggs. Egg shape index has a great influence on the hatchab...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11G16B20/20
CPCC12Q1/6888G16B20/20C12Q2600/124C12Q2600/156
Inventor 杨秀荣邓继贤邹乐勤
Owner GUANGXI UNIV
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