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Mycoplasma ovipneumoniae, A-type sheep pasteurella multocida and D-type sheep pasteurella multocida triple inactivated vaccine

A technology of Mycoplasma pneumoniae and Pasteurella, applied in the field of vaccines, can solve the problems of unsatisfactory immune effect and aggravate the pathogenesis of sheep infectious pleuropneumonia, and achieve the effect of reducing stress and cost of sheep

Active Publication Date: 2020-08-11
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The measure of prevention sheep contagious pleuropneumonia in the prior art is mainly to inoculate immunization Mycoplasma ovis pneumonia inactivated single vaccine, but because Mycoplasma ovis pneumonia is often mixed with A-type Pasteurella multocida and D-type Pasteurella multocida Mixed infection, and Pasteurella multocida type A and Pasteurella multocida type D will cause Pasteurella multocida disease in sheep, and then aggravate the pathogenesis of sheep infectious pleuropneumonia, resulting in the current commercially available Immune effect of inactivated single vaccine of Mycoplasma pneumoniae in sheep is not satisfactory

Method used

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  • Mycoplasma ovipneumoniae, A-type sheep pasteurella multocida and D-type sheep pasteurella multocida triple inactivated vaccine
  • Mycoplasma ovipneumoniae, A-type sheep pasteurella multocida and D-type sheep pasteurella multocida triple inactivated vaccine
  • Mycoplasma ovipneumoniae, A-type sheep pasteurella multocida and D-type sheep pasteurella multocida triple inactivated vaccine

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preparation example Construction

[0038] The present invention provides a preparation method of the triple inactivated vaccine described in the above scheme, comprising the following steps:

[0039] 1) Culture Mycoplasma ovis pneumoniae MO_NM01, Pasteurella multocida type A PM-NM1, Pasteurella multocida type D P<-NM2 respectively, and prepare Mycoplasma pneumoniae culture medium and Type A ovine multocida respectively. Sexual Pasteurella bacteria liquid and D-type Pasteurella multocida bacteria liquid;

[0040] 2) Inactivate the Mycoplasma ovis pneumoniae MO_NM01 bacterial liquid, the Pasteurella multocida type A sheep PM-NM1 bacterial liquid and the Pasteurella multocida type D sheep P<-NM2 bacterial liquid respectively and mix them to obtain inactivation microbial agents;

[0041] 3) Mixing the inactivated microbial preparation and the immune adjuvant to obtain a triple inactivated vaccine.

[0042] In the present invention, firstly culture Mycoplasma ovis pneumoniae MO_NM01, Pasteurella multocida type A s...

Embodiment 1

[0093] Example 1 Preparation of Mycoplasma ovis pneumoniae MO_NM01, Pasteurella multocida type A and Pasteurella multocida type D "ISA-201-VG" compound oil adjuvant triple inactivated vaccine

[0094] 1. Production strain preparation of Mycoplasma ovis pneumoniae MO_NM01

[0095] 1 Production strain preparation

[0096] 1.1 First-class seed propagation

[0097] Freeze-dried strains of Mycoplasma ovis pneumoniae MO_NM01 were streaked and inoculated on Mycoplasma solid medium On the plate, cultured at 37°C for 72 hours, picked a single colony in Improved KM2 liquid medium cultured at 37°C for 48 hours to obtain first-class seeds of Mycoplasma ovis pneumoniae MO_NM01.

[0098] 1.2 Secondary seed propagation

[0099] Insert the prepared first-class seeds of Mycoplasma ovis pneumoniae MO_NM01 at a volume ratio of 1% Improved KM2 Liquid In the culture medium, cultivate at 37°C for 48 hours, conduct a pure inspection according to the appendix of the current "Chinese Veterinary...

Embodiment 2

[0128] Example 2 Preparation of triple inactivated vaccine with Freund's adjuvant for Mycoplasma ovis pneumoniae, Pasteurella multocida type A and Pasteurella multocida type D

[0129] 1. The equal volume obtained in Example 1, the concentration is 2×10 9 CFU / mL of Pasteurella multocida type A sheep PM-NM1 bacterial liquid and Pasteurella multocida type D sheep P9 CCU / mL Mycoplasma ovis pneumoniae MO_NM01 bacterium liquid resuspends the above-mentioned two kinds of Pasteurella precipitation, and the concentration of two kinds of Pasteurella bacterium liquids in the obtained resuspension liquid is also respectively 2 * 10 9 CFU / ml; then add the same volume as the resuspension Freund's adjuvant , to obtain a triple inactivated vaccine; the bacterium concentration of Mycoplasma ovis pneumoniae MO_NM01 in the triple inactivated vaccine is 1×10 9 CCU / mL, the cell concentration of Pasteurella multocida type A sheep PM-NM1 is 1×10 9 CFU / mL, the concentration of Pasteurella multoci...

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Abstract

The invention provides a mycoplasma ovipneumoniae, A-type pasteurella multocida and D-type pasteurella multocida triple inactivated vaccine, and belongs to the technical field of vaccines. The tripleinactivated vaccine comprises inactivated bacterium liquid of mycoplasma ovipneumoniae MO_NM01, inactivated bacterium liquid of A-type sheep pasteurella multocida PM-NM1, inactivated bacterium liquidof D-type sheep pasteurella multocida P<-NM2 and an immunologic adjuvant. Compared with commercially available mycoplasma ovipneumoniae inactivated vaccines, the mycoplasma ovipneumoniae, A-type pasteurella multocida and D-type pasteurella multocida triple inactivated vaccine provided by the invention has basically equivalent immune protection force against corresponding pathogens, but can achievethe purposes of multiple prevention by one injection, cost reduction and sheep stress reduction.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a triple inactivated vaccine of Mycoplasma ovis pneumoniae, Pasteurella multocida type A and Pasteurella multocida type D. Background technique [0002] Mycoplasma ovis, Pasteurella multocida type A and Pasteurella multocida type D are the most important conditional pathogens that endanger the sheep industry today. Secondary infection of pathogens seriously endangers the healthy and sustainable development of sheep industry in my country and even in the world. [0003] The measure of prevention sheep contagious pleuropneumonia in the prior art is mainly to inoculate immunization Mycoplasma ovis pneumonia inactivated single vaccine, but because Mycoplasma ovis pneumonia is often mixed with A-type Pasteurella multocida and D-type Pasteurella multocida Mixed infection, and Pasteurella multocida type A and Pasteurella multocida type D will cause Pasteurella multocida disease in shee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61K39/102A61P31/04A61P11/00
CPCA61K39/0241A61K39/102A61K2039/521A61K2039/541A61K2039/552A61K2039/70A61P11/00A61P31/04A61K2300/00
Inventor 张月梅赵世华戴伶俐王娜宋越张帆周蕾达来宝力格刘威杨斌柴春霞白帆陈伟宋爱军
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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