Antibacterial composition, antibacterial composite nanoparticle, preparation method of antibacterial composite nanoparticle and targeted sustained-release private nanometer gel
A technology of composite nanoparticles and antibacterial composition, applied in the field of personal care, can solve the problems of cell body damage, unfavorable dispersion, use restriction, etc., and achieve the effect of prolonging the action time
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[0030] The present invention also provides a method for preparing antibacterial composite nanoparticles, comprising: S1) mixing the antibacterial composition with water to obtain an antibacterial composition suspension; the antibacterial composition includes Bacillus subtilis fermentation broth, cranberry extract compound and dandelion extract; polylactic acid is mixed with an organic solvent to obtain a polylactic acid organic solution; S2) adding the suspension of the antibacterial composition dropwise to the polylactic acid organic solution, after ultrasonication, centrifugation, and freeze-drying, to obtain an antibacterial composite nanoparticles.
[0031] Among them, the present invention has no special limitation on the sources of all raw materials, which can be commercially available.
[0032] The antibacterial composition is mixed with water to obtain an antibacterial composition suspension; the water is preferably ultrapure water; the ratio of the antibacterial compo...
Embodiment 1
[0047] Example 1: Preparation of polylactic acid-coated antibacterial composite nanoparticles
[0048] Weigh 0.5 mg of Bacillus subtilis fermentation broth, 0.3 mg of cranberry extract, and 0.1 mg of dandelion extract and place them in 1ml of ultrapure water, set the ultrasonic power to 2500W, and the temperature to 30°C for 25 minutes in a water bath to make Its mixed solution is evenly dispersed to form a uniform suspension; 30 mg of polylactic acid (weight average molecular weight 1W, viscosity 0.25dl / g) is weighed and dissolved in 10 ml of acetone to form a polylactic acid acetone solution; the prepared uniform suspension is gradually Add it dropwise into the polylactic acid acetone solution, after ultrasonication for 25min (ultrasonic power is 2500W), then collect it under the centrifugation speed of 2500r / min for 15min, wash with ultrapure water, and freeze-dry to obtain polylactic acid-wrapped antibacterial compound Nanoparticles1.
Embodiment 2
[0049] Example 2: Preparation of polylactic acid-wrapped antibacterial composite nanoparticles
[0050] Weigh 1 mg of Bacillus subtilis fermentation broth, 0.8 mg of cranberry extract, and 0.5 mg of dandelion extract, put them in 2ml of ultrapure water, set the ultrasonic power to 2800W, and set the ultrasonic temperature to 30°C for 27 minutes in a water bath to make it The mixed solution is uniformly dispersed to form a uniform suspension; weigh 40 mg of polylactic acid (weight average molecular weight 1W, viscosity 0.25dl / g) and dissolve it in 15ml with stirring to make a polylactic acid acetone solution, and add the obtained uniform suspension dropwise to In polylactic acid acetone solution, after ultrasonication for 30min (ultrasonic power is 2500W), it is collected under the centrifugal speed of 2800r / min for 15min, washed with ultrapure water, and freeze-dried to obtain polylactic acid-wrapped antibacterial composite nanoparticles 2.
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