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Novel coronavirus antibody joint detection kit and application thereof

A coronavirus and combined detection technology, applied in the field of combined detection kits for new coronavirus antibodies, can solve the problems of high laboratory standards, limited nucleic acid detection, aerosol pollution, etc., and achieves convenient use, high sensitivity and specificity. Sexual, receptive effects

Active Publication Date: 2020-08-07
厦门万渤生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing detection methods are based on traditional fluorescent PCR reagents. Due to the complicated operation, the possible generation of aerosols that may cause large-scale pollution (produce false positive samples), high requirements for laboratory standards, and long detection time, etc., the current nucleic acid Testing is still very limited

Method used

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  • Novel coronavirus antibody joint detection kit and application thereof
  • Novel coronavirus antibody joint detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0028] A joint detection kit for novel coronavirus antibody, which includes a diluent, and the novel coronavirus IgM / IgG joint test strip includes a base plate and sequentially connected sample pads, binding pads, reaction membranes, and water-absorbing pads arranged on the base plate The reaction membrane is provided with a first detection area, a second detection area and a quality control area, and the binding pad is coated with a recombinant SARS-COV-2 antigen with a chromogenic label and a mouse IgG antibody with a chromogenic label. The detection area is coated with mouse anti-human IgM monoclonal antibody, the second detection area is coated with mouse anti-human IgG monoclonal antibody, and the quality control area is coated with goat anti-mouse IgG antibody.

[0029] When in use, after dripping or dipping the sample solution into the sample pad, let it stand for 5-10 minutes, and observe the color development of the first detection area, the second detection area and t...

Embodiment approach 2

[0050] This embodiment lies in the application of the detection kit in Embodiment 1 in the detection of IgG and IgM of the novel coronavirus in whole blood, serum or plasma.

[0051] Further, after the sample is placed on the sample pad, the diluent is added, and the color reaction of the quality control area, the first detection area and the second detection area is observed at rest.

Embodiment 1

[0054] Such as figure 1 As shown, the combined detection kit of novel coronavirus antibody includes a test strip 1, and the test strip includes a bottom plate and a sample pad 12, a binding pad 13, a reaction membrane 14, and a water-absorbent pad 15 connected in sequence on the bottom plate; The reaction membrane 14 is provided with a first detection zone 141, a second detection zone 142 and a quality control zone 143, and the binding pad 13 is coated with a recombinant SARS-COV-2 antigen with a chromogenic marker and a chromogenic marker. For mouse IgG antibody, the first detection area 141 is coated with mouse anti-human IgM monoclonal antibody, the second detection area 142 is coated with mouse anti-human IgG monoclonal antibody, and the quality control area 143 is coated with goat anti-mouse IgG antibody.

[0055] Recombinant SARS-COV-2N protein antigen, using genetic engineering technology to construct a plasmid, containing his-tag tag, soluble expression.

[0056] Goat...

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Abstract

The invention relates to the detection of a coronavirus antibody, in particular to a novel coronavirus antibody combined detection kit and the application thereof. The kit comprises a diluent and a novel coronavirus IgM / IgG combined test strip, and the novel coronavirus IgM / IgG combined test strip comprises a bottom plate, and a sample pad, a combination pad, a reaction film and a water absorptionpad which are arranged on the bottom plate and connected in sequence. A first detection area, a second detection area and a quality control area are arranged on the reaction film, the conjugate pad is coated with a recombinant SARS-COV-2 antigen with a chromogenic marker and a mouse IgG antibody with a chromogenic marker, the first detection area is coated with a mouse anti-human IgM monoclonal antibody, the second detection area is coated with a mouse anti-human IgG monoclonal antibody, and the quality control area is coated with a goat anti-mouse IgG antibody. According to the invention, the IgM and IgG can be jointly determined without a special device, and the kit has higher sensitivity and is of great significance to the detection of early infection and long-term infection.

Description

technical field [0001] The invention relates to the detection of a coronavirus antibody, in particular to a combined detection kit for a novel coronavirus antibody and its application. Background technique [0002] SARS-CoV-2 is an acute respiratory infectious disease, mainly caused by 2019 novel coronavirus (2019-nCoV) infection. The transmission routes are mainly direct transmission, aerosol transmission and contact transmission. The new type of coronavirus pneumonia is a typical emergency disease, and the clinic needs to know the test results as soon as possible so that the next step of prevention and control measures can be taken. However, most of the existing detection methods are based on traditional fluorescent PCR reagents. Due to the complicated operation, the possible generation of aerosols causing large-scale pollution (generating false positive samples), high requirements for laboratory standards, and long detection time, etc., the current nucleic acid Testing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/569G01N33/558
CPCG01N33/6854G01N33/577G01N33/56983G01N33/558G01N2469/20G01N2333/165Y02A50/30
Inventor 范文丽
Owner 厦门万渤生物技术有限公司
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