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Method for extracting nucleotide and polypeptide from animal viscera

A technology for animal viscera and nucleotides, applied in the field of extracting nucleotides and peptides from animal viscera, can solve the problems of complicated and wasteful procedures, and achieve the effect of strong adsorption force, affinity and high efficiency

Pending Publication Date: 2020-08-07
郑春发
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Animal viscera is rich in nucleotides and polypeptides, but currently the extraction of nucleotides and polypeptides is mostly carried out separately, resulting in waste of resources and complicated procedures. Based on this, this application proposes a Methods for Extracting Nucleotides and Peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting nucleotides and polypeptides from animal viscera, comprising the following steps:

[0024] S1, take fresh animal viscera, wash, drain, chop and then mash, add deionized water to obtain a mixed solution, wherein the quality of the deionized water is 12 times that of fresh animal viscera;

[0025] S2, add the solution of guanidine isothiocyanate to the mixed solution, the concentration of the guanidine isothiocyanate solution is 4mol / L, and the mass ratio to the mixed solution is 1:0.1, vibrate and mix well and let stand for 1 Hour;

[0026] S3, adding a carrier containing silica to it, the mass ratio of the carrier containing silica to the solution is 0.1:1, the mass ratio of silica in the carrier is not less than 7%, vibrate and mix well and let stand for a period of time For 0.5 hours, guanidine isothiocyanate lyses the cells, and the nucleotides released from the cells bind to the silica;

[0027] S4, taking out the carrier containing silicon...

Embodiment 2

[0032] A method for extracting nucleotides and polypeptides from animal viscera, comprising the following steps:

[0033] S1, take the fresh animal offal, wash it, drain it, chop it and then mash it, add deionized water to obtain a mixed solution, wherein the quality of the deionized water is 15 times that of the fresh animal offal;

[0034] S2, add the solution of guanidine isothiocyanate to the mixed solution, the concentration of the guanidine isothiocyanate solution is 4mol / L, and the mass ratio to the mixed solution is 1:0.15, vibrate and mix well and let stand for 1 Hour;

[0035] S3, adding a carrier containing silica to it, the mass ratio of the carrier containing silica to the solution is 0.15:1, the mass ratio of silica in the carrier is not less than 7%, vibrate and mix well and let it stand for a period of time For 0.5 hours, guanidine isothiocyanate lyses the cells, and the nucleotides released from the cells bind to the silica;

[0036] S4, taking out the carri...

Embodiment 3

[0041] A method for extracting nucleotides and polypeptides from animal viscera, comprising the following steps:

[0042] S1, taking fresh animal offal, washing it, draining it, chopping it and mashing it, adding deionized water to obtain a mixed solution, wherein the quality of the deionized water is 13 times that of the fresh animal offal;

[0043] S2, add the solution of guanidine isothiocyanate to the mixed solution, the concentration of the guanidine isothiocyanate solution is 4mol / L, and the mass ratio to the mixed solution is 1:0.13, vibrate and mix well and let stand for 1 Hour;

[0044] S3, adding a carrier containing silica to it, the mass ratio of the carrier containing silica to the solution is 0.13:1, the mass ratio of silica in the carrier is not less than 7%, vibrate and mix, and then stand still for a period of time For 0.5 hours, guanidine isothiocyanate lyses the cells, and the nucleotides released from the cells bind to the silica;

[0045] S4, taking out ...

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PUM

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Abstract

The invention discloses a method for extracting nucleotide and polypeptide from animal viscera. The method comprises the following steps of: taking fresh animal viscera, performing cleaning, draining,chopping and mashing, and adding deionized water to obtain a mixed solution; adding a guanidinium isothiocyanate solution into the mixed solution, vibrating the solution to mix the substances uniformly, and then performing standing; adding a carrier containing silicon dioxide into the solution, performing vibration and mixing the substances uniformly, then conducting standing, cracking cells by guanidinium isothiocyanate, and binding nucleotide released from the cells to silicon dioxide; taking out the carrier containing silicon dioxide, leaving the remaining solution for later use, carryingout adsorption and desorption on the carrier by an eluent, and conducting separation to obtain purified nucleotide. The invention has the beneficial effects that: the polypeptide and nucleotide can beextracted in sequence, nucleotide is extracted in a silicon dioxide adsorption mode, the carrier only has high adsorption capacity and affinity to nucleotide and does not adsorb other biochemical components like polysaccharide, and meanwhile the problem that mutual reaction between biochemical reagents when the biochemical reagents are added for extraction can be avoided.

Description

technical field [0001] The invention relates to the technical field of extracting nucleotides and polypeptides, in particular to a method for extracting nucleotides and polypeptides from animal viscera. Background technique [0002] Nucleotides are a class of compounds composed of purine bases or pyrimidine bases, ribose or deoxyribose sugars, and phosphoric acid. They are widely used in medicine, maternal and child products, condiments, and food additives. Polypeptide is a compound formed by α-amino acids linked together by peptide bonds. It is an intermediate product of protein hydrolysis. Peptides are involved in various fields of human hormones, nerves, cell growth and reproduction, and their importance lies in regulating the physiology of various systems and cells in the body. function, activate relevant enzyme systems in the body, promote the permeability of intermediate metabolic membranes, or control DNA transcription or affect specific protein synthesis, and finally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07H19/00C07H21/00C07H1/08
CPCC12P21/06C07K1/14C07H19/00C07H21/00C07H1/08
Inventor 郑春发马常升
Owner 郑春发
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