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Method for producing rose hairy roots through induction of agrobacterium rhizogenes

A technology of Agrobacterium rhizogenes and hairy roots, applied in the field of plant biotechnology engineering, can solve the problems of inability to use medicinal ingredients on a large scale, scarcity and extraction of wild rose plants, and achieve good development and application prospects

Pending Publication Date: 2020-08-07
YANGZHOU UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to propose a method for inducing hairy roots of roses, that is, a method for inducing hairy roots of roses by using Agrobacterium rhizogenes, and utilizing the characteristics of fast growth of hairy roots to solve the problem that wild rose plants are scarce and cannot be produced on a large scale. Technical issues for the extraction of medicinal ingredients

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  • Method for producing rose hairy roots through induction of agrobacterium rhizogenes
  • Method for producing rose hairy roots through induction of agrobacterium rhizogenes
  • Method for producing rose hairy roots through induction of agrobacterium rhizogenes

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Embodiment 1

[0026] 1. Preservation and activation of Agrobacterium rhizogenes (strain ATCC15834)

[0027] Long-term storage: use 20% (volume concentration) glycerin for storage, that is, add glycerin and bacterial liquid at a volume ratio of 1:4, mix well, and store in a -80°C refrigerator. This method can preserve bacteria for 3 to 5 years.

[0028] Activation of long-term preserved strains: In the ultra-clean workbench, pick a well-growing single colony from the glycerin-preserved strains, streak it on the YEB solid medium, and place it upside down in a constant temperature incubator at 28°C for about 36 hours, until more colonies with a diameter of about 1cm grow, pick a single colony in 3mL YEB liquid medium, culture for 12~14h, draw 30μL of activated bacteria liquid and connect it to 30mL YEB liquid medium, 200rpm Shake culture for 12-14 hours, repeat the above steps for subculture 3-4 times, and store the activated bacterial liquid in a refrigerator at 4°C. The culture conditions a...

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Abstract

The invention relates to a method for producing rose hairy roots through induction of agrobacterium rhizogenes, and belongs to the field of plant biotechnology engineering. The method comprises the steps of agrobacterium rhizogenes preservation and activation, infection liquid preparation, explant pre-culture and co-culture, infection and induction of hairy roots by using agrobacterium tumefaciens, and liquid multiplication culture on hairy roots. By utilizing the characteristic of a high growth speed of hairy roots, the technical problem that wild rose plants are scarce and cannot be used forextracting medicinal components on a large scale is solved.

Description

technical field [0001] The invention belongs to the field of plant biotechnology engineering, and relates to a method for cultivating hairy roots of roses (Rosa rugosa Thunb.), in particular to a method for inducing hairy roots of roses by using Agrobacterium rhizogenes. Background technique [0002] Rose (Rosa rugosa Thunb.) is an upright shrub belonging to the genus Rosa in the family Rosaceae. Because of its high content of essential oil, good quality and international fragrance, the cultivation area is constantly expanding. In recent years, the medicinal value of rose has been paid more and more attention. On the one hand, it is recorded in the Pharmacopoeia that roses can relieve pain, reduce swelling, promote blood circulation and remove blood stasis. oxidation. The latest research in phytochemistry has found that the high content of rosin in rose roots has important medicinal value: it can promote the scavenging of oxygen free radicals in cardiomyocytes in a hypobari...

Claims

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Application Information

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IPC IPC(8): C12N15/84A01H5/06A01H6/74
CPCC12N15/8205A01H4/005
Inventor 冯立国曾雨倩王建文
Owner YANGZHOU UNIV
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