Chimeric molecule for mediating mitochondrial targeted degradation based on autophagy mechanism and application thereof

Abstract

The invention discloses a chimeric molecule for mediating mitochondrial targeted degradation based on autophagy mechanism. The amino acid sequence of the chimeric molecule is shown by SEQ ID NO: 1 or2 or 3 or 4. The invention also discloses a nucleic acid molecule for encoding the chimeric molecule for mediating mitochondrial targeted degradation based on, an expression vector containing the nucleic acid molecule, and applications of the chimeric molecule, the nucleic acid molecule and the expression vector in targeted degradation of mitochondria. According to the chimeric molecule for mediating mitochondrial targeted degradation, through targeted degradation of intracellular mitochondria, it is proved that regulation and control of intracellular organelles at the cellular level are feasible, and reference is provided for targeted degradation of other organelles; the chimeric molecule disclosed realizes degradation of the intracellular mitochondria at a cellular level, and provides adrug research and development thought for treatment of diseases; and particularly, a certain treatment thought can also be provided for neurodegenerative diseases caused by PINK1-Parkin pathway mutation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chimeric molecule that mediates mitochondrial targeted degradation based on an autophagy mechanism and an application thereof. Background technique [0002] Mitochondria are ubiquitous organelles in eukaryotic cells that participate in respiration and energy metabolism, and are the most important energy source for various physiological activities in cells. Most cells in the human body obtain the energy needed to maintain their own metabolism through the oxidative phosphorylation of mitochondria. [0003] Mitochondria are organelles in constant dynamic change. Mitochondria realize the self-renewal of mitochondria through fusion and fission, as well as the process of mitophagy, so as to ensure the normal progress of various physiological functions of mitochondria. Mitophagy is a type of selective autophagy that is primarily responsible for the removal of dysfunctional or damaged mi...

AI Technical Summary

Problems solved by technology

However, these methods cannot directly regulate the level of intracellular mitochondria, and the use of traditional drugs will also cause serious side effects

Images