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Method for producing soybean peptone by using puffed soybean protein base material and soybean peptone

A technology of soybean peptone and soybean protein, which is applied in the field of soybean peptone, can solve the problems of poor solubility, poor dispersibility, and long enzymatic hydrolysis time, and achieves the advantages of rapid growth and reproduction, good enzymatic hydrolysis effect, and enzymatic hydrolysis time. short effect

Pending Publication Date: 2020-07-28
PINGLIANG HUAKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still problems of long enzymatic hydrolysis, poor solubility and poor dispersibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method utilizing expanded soybean protein base material to produce soybean peptone, comprising the following steps:

[0027] S1: Take the soybean protein isolate base material and perform expansion treatment on it; the pressure of the extrusion expansion is 3-8Mpa, the temperature is 180-220°C, and the treatment time is 23min.

[0028] S2: the expanded soy protein isolate base material and water are configured into a dispersion liquid that is 15% by weight;

[0029] S3: adjust the pH value of the dispersion to 7, add protease, the percentage by weight of the dry basis of the soybean protein isolate base material after protease and puffing is 5%, at a temperature of 40-70°C, enzymolyze for 36min, and then sterilize Enzyme treatment;

[0030] S4: filter through a plate and frame filter press or membrane separation, purification and refinement, spray dry after double-effect concentration, and obtain soybean peptone.

[0031] The soybean peptone prepared above has a pro...

Embodiment 2

[0033] A method utilizing expanded soybean protein base material to produce soybean peptone, comprising the following steps:

[0034] S1: Take the soybean protein isolate base material and perform expansion treatment on it; the pressure of air expansion is 0.8-1.25Mpa, the temperature is 110-130°C, and the treatment time is 16min.

[0035] S2: the expanded soy protein isolate base material and water are configured into a dispersion liquid that is 12% by weight;

[0036] S3: adjust the pH value of the dispersion to 9, add protease, the percentage by weight of the dry basis of the soybean protein isolate base material after protease and puffing is 5%, at a temperature of 40-70°C, enzymolyze for 38min, and then sterilize Enzyme treatment;

[0037] S4: filter through a plate and frame filter press or membrane separation, purification and refinement, spray dry after double-effect concentration, and obtain soybean peptone.

[0038] The soybean peptone prepared above has a protein ...

Embodiment 3

[0040] A method utilizing expanded soybean protein base material to produce soybean peptone, comprising the following steps:

[0041] S1: Take soybean protein isolate base material and perform puffing treatment on it; the microwave puffing temperature is about 78-85° C., and the treatment time is 4 minutes.

[0042] S2: the expanded soy protein isolate base material and water are configured into a dispersion liquid that is 16% by weight;

[0043] S3: adjust the pH value of the dispersion to 9, add protease, the percentage by weight of the dry basis of the soybean protein isolate base material after protease and puffing is 5%, at a temperature of 40-70°C, enzymolyze for 32min, and then sterilize Enzyme treatment;

[0044] S4: filter through a plate and frame filter press or membrane separation, purification and refinement, spray dry after double-effect concentration, and obtain soybean peptone.

[0045] The soybean peptone prepared above has a protein content of 89%; contains...

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PUM

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Abstract

The invention discloses a method for producing soybean peptone by using a puffed soybean protein base material and the soybean peptone. The method comprises the following steps: taking a soybean protein isolate base material and puffing the soybean protein isolate base material; preparing a dispersion liquid with a weight percentage of 5-20% from the puffed soybean protein isolate base material and water; adjusting the pH value of the dispersion liquid to 6.0-10.0, adding protease, wherein the weight percentage of the protease and the dry base of the puffed soybean protein isolate base material is 0.1-8%, performing enzymolysis for 30-50 min at the temperature of 40-70 DEG C, and then carrying out enzyme deactivation treatment; and filtering through a plate-and-frame filter press or purifying and refining through membrane separation, and carrying out spray drying after double-effect concentration to obtain the soybean peptone. According to the process method disclosed by the invention,the obtained peptone product is easy to dissolve in water, high in nutritional value and good in dispersity.

Description

technical field [0001] The invention relates to the production field of soybean peptone, in particular to a method for producing soybean peptone by using expanded soybean protein base material and the soybean peptone. Background technique [0002] Peptone is a water-soluble mixture obtained by enzymatic hydrolysis of protein, mainly composed of peptone, peptide and amino acid. Peptone can be divided into animal peptone, plant peptone and microbial peptone in terms of source. The traditional fermentation industry, antibiotic companies and some biopharmaceutical companies mainly use casein as the source of peptone for daily production. However, animal-derived peptone has obvious disadvantages, such as microbial contamination such as prions, large batch-to-batch differences, unclear components, and unfavorable separation and purification of target products such as vaccines and monoclonal antibodies. In the field of microbial fermentation, especially in the biopharmaceutical i...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P13/04C12N1/20C12R1/01
CPCC12P21/06C12P13/04C12N1/20
Inventor 员斌
Owner PINGLIANG HUAKE BIOLOGICAL TECH
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