Kit for rapidly capturing, releasing and detecting circulating tumor cells without damage

Abstract

The invention discloses a kit for rapidly capturing, releasing and detecting circulating tumor cells without damage, and relates to the field of biotechnology and biomedicine. The kit includes a CTCsmagnetic nano capture probe solution, an erythrocyte lysis solution, a CTCs releasing solution, a CTCs detecting solution and washing buffer; CTCs magnetic nano capture probes are the magnetic nanoparticles whose surfaces are modified with folic acid; the folic acid on the CTCs magnetic nano capture probes specifically bind to the folate receptors on the surfaces of the circulating tumor cells, sothat the capturing and separation of the circulating tumor cells can be realized at an external magnetic field; and the CTCs detecting solution can perform fluorescence labeling on the released CTCs,so that observation and counting can be realized under a fluorescence microscope. The CTCs captured and released by the kit retain very high activity, so that the kit has significant meaning on the early in vitro screening and diagnosis of cancer and the follow-up study of the CTCs; and the components in the kit can be selectively used according to specific demands, so that simple and convenientoperation can be realized, and therefore, the kit can have wide application prospects.

Description

technical field [0001] The invention relates to the fields of biotechnology and biomedicine, in particular to a rapid and non-damaging capture, release and detection kit for circulating tumor cells. Background technique [0002] Circulating tumor cells (CTCs) are important biomarkers in tumor liquid biopsy. CTCs refer to the general term for tumor cells that have shed from the primary tumor or metastases into the peripheral blood. CTCs appear earlier than solid tumors visible on imaging, and can be used as markers for early diagnosis of tumors. In addition, the number and phenotype of CTCs are closely related to tumor progression, metastasis and prognosis. Clinical detection and counting of CTCs can be used for dynamic monitoring of tumor occurrence and development, evaluation of tumor treatment effects, and guidance for the formulation and implementation of individualized tumor treatment plans. However, the content of CTCs is extremely rare, and its detection first needs...

AI Technical Summary

Problems solved by technology

However, this method has certain limitations: 1) The high cost of antibodies, poor stability, and short shelf life make immunomagnetic separation costly; 2) The process of tumor metastasis to form CTCs will undergo epithelial-mesenchymal transition (EMT) , Epithelial-mesenchymal transition leads to down-regulation or even non-expression of EpCAM on the cell surface, making it easy to miss mesenchymal CTCs by EpCAM-based immunomagnetic separation methods; 3) Based on antigen-antibody recognition, magnetic beads will bind to CTCs 4) Magnetic beads bound to the surface of CTCs will eventually be internalized by CTCs, resulting in cytotoxicity and affecting the true properties of CTCs, etc.

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