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Chemiluminescence sensor for detecting acetamiprid and preparation method of sensor

A chemiluminescence and chemiluminescence detection technology, which is applied in the field of sensors, can solve the problems of complex operation, time-consuming and laborious, etc., and achieve the effects of fast detection speed, high sensitivity detection, and improved reaction speed

Active Publication Date: 2020-07-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the disadvantages of complex, time-consuming and labor-intensive operations and the need for professional technicians in the existing detection methods, and provides a chemiluminescence biosensor with high sensitivity, strong specificity, low cost and rapid detection of acetamiprid

Method used

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  • Chemiluminescence sensor for detecting acetamiprid and preparation method of sensor
  • Chemiluminescence sensor for detecting acetamiprid and preparation method of sensor
  • Chemiluminescence sensor for detecting acetamiprid and preparation method of sensor

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Embodiment 1

[0057] The preparation method of described biosensor comprises the following steps:

[0058] (1) Preparation of gold nanoparticles;

[0059] (2) Modification of closed Walker chains and HAP chains containing -SH onto the surface of gold nanoparticles;

[0060] (3) Homogeneous reaction of labeled gold nanoparticles;

[0061] (4) Chemiluminescence detection.

[0062] The present invention entails the synthesis of gold nanoparticles for use as carriers for DNA Walkers.

[0063] The preparation method operation steps of described gold nanoparticles are as follows:

[0064] It was prepared by the trisodium citrate reduction method. Before the synthesis, the glass instruments used had to be soaked in aqua regia for 24 hours and rinsed with ultrapure water. Then, 500 µL 0.04g·mL -1 HAuCl 4 Add to a three-neck flask containing 200mL of ultrapure water, and heat to boiling. Under stirring conditions, quickly add 3mL of 1% trisodium citrate solution, within a few minutes, the col...

Embodiment 2

[0077] A preparation method of the fluorescent biosensor of the present invention:

[0078] The preparation method of described biosensor comprises the following steps:

[0079] (1) Preparation of gold nanoparticles;

[0080] (2) Modification of closed Walker chains and HAP chains containing -SH onto the surface of gold nanoparticles;

[0081] (3) Homogeneous reaction of labeled gold nanoparticles;

[0082] (4) Chemiluminescence detection.

[0083] The present invention entails the synthesis of gold nanoparticles for use as carriers for DNA Walkers.

[0084] The preparation method operation steps of described gold nanoparticles are as follows:

[0085] It was prepared by the trisodium citrate reduction method. Before the synthesis, the glass instruments used had to be soaked in aqua regia for 24 hours and rinsed with ultrapure water. Then, 500 µL 0.04g·mL -1 HAuCl 4 Add to a three-neck flask containing 200mL of ultrapure water, and heat to boiling. Under stirring condi...

Embodiment 3

[0099] The preparation method of described biosensor comprises the following steps:

[0100] (1) Preparation of gold nanoparticles;

[0101] (2) Modification of closed Walker chains and HAP chains containing -SH onto the surface of gold nanoparticles;

[0102] (3) Homogeneous reaction of labeled gold nanoparticles;

[0103] (4) Chemiluminescence detection.

[0104] The present invention entails the synthesis of gold nanoparticles for use as carriers for DNA Walkers.

[0105] The preparation method operation steps of described gold nanoparticles are as follows:

[0106] It was prepared by the trisodium citrate reduction method. Before the synthesis, the glass instruments used had to be soaked in aqua regia for 24 hours and rinsed with ultrapure water. Then, 500 µL 0.04g·mL -1 HAuCl 4 Add to a three-neck flask containing 200mL of ultrapure water, and heat to boiling. Under stirring conditions, quickly add 3mL of 1% trisodium citrate solution, within a few minutes, the col...

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Abstract

The invention relates to the technical field of biosensors, in particular to a chemical biosensor using a DNAzyme based DNA Walker, aims to solve the problems of relatively low specificity and sensitivity and high cost of acetamiprid detection methods in the prior art, and discloses a chemical biosensor for detecting acetamiprid by the DNAzyme based DNA Walker. DNAzyme cracking is used for assisting in realizing circulating amplification and forming a lot of G-quadruplets simulating the horseradish peroxidase activity on gold nanoparticles. A preparation method comprises the following steps ofpreparing the gold nanoparticles; modifying a Walker chain and a Lock chain to the surfaces of the gold nanoparticles; producing a homogeneous reaction of a labeled gold nanoparticle solution; carrying out a DNAzyme cracking reaction, and chemiluminiscence detection; carrying out high-specificity detection on the acetamiprid as a target object by using a nucleic acid aptamer; and realizing the signal amplification effect by utilizing DNAzyme cracking reaction amplification.

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to a chemiluminescence sensor for detecting acetamiprid based on a DNAzyme DNA Walker, and also relates to a preparation method thereof. Background technique [0002] Acetamiprid, as a neonicotinoid insecticide, is widely used in agricultural production because it has no cross-resistance to common organophosphorus and carbamate pesticides. Once applied, acetamiprid is mainly taken up by the roots and then transferred to various tissues of the organism, including flowers, leaves and fruits. From the perspective of pest control, compared with other insecticides, acetamiprid has the advantages of better absorption rate and ease of use. However, for reasons of human exposure and eco-friendliness, acetamiprid will undoubtedly affect non-target organisms that may come into contact with plant production. The use of acetamiprid has been banned in many countries due to insecticide resistan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/682C12Q1/6825C12Q1/44G01N21/76
CPCC12Q1/682C12Q1/6825C12Q1/44G01N21/76C12Q2525/205C12Q2521/345C12Q2525/301C12Q2563/137C12Q2563/103C12Q2565/607
Inventor 刘素张曼茹王玉黄加栋王敬锋张儒峰赵一菡瞿晓南孙文玉王业茹江龙
Owner UNIV OF JINAN
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